RT Conference Proceedings
T1 Bacterial contact induces morphological and functional changes in primary neural cortical cell cultures
A1 Lombardo Hernández, Juan
A1 Muñoz Rodríguez, David
A1 Bourquia Ramzi, Marwane
A1 García Perez, Pablo
A1 Murciano Cespedosa, Antonio
A1 Geuna, Stefano
A1 Conejero Meca, Francisco José
A1 Mateos González, Álvaro
A1 García Esteban, María Teresa
A1 Herrera Rincón, Celia
AB The interaction of bacteria with various somatic cell types is an exciting emerging field. Despite the known effects of microbiota on the gut-brain axis, very little is known about the direct interactions that bacteria could have with neurons, both in terms of molecular mechanisms and information transfer. In order to study these communication mechanisms, this study designs an in vitro model to co-cultivate microbiota-bacteria Lactiplantibacillus plantarum with neural cortical cells and analyzes the effects of this process in both populations. Here, we show how bacteria and neurons can be co-cultured, and demonstrate a novel integrated platform that facilitates the analysis of neuronal-bacteria communication. The results we obtained showed that L.plantarum is capable of adhering to the surface of the neural culture and the amount of attached bacteria increases with co-culture time. In addition, neural co-cultured cells show morphological and functional changes in the expression of key proteins in neuroplasticity such as Synapsin I and pCREB. Finally, using real-time optical (calcium signaling) readouts, we show that neural cells react to the co-culture with bacteria by increasing cytoplasmatic Ca2+ signaling. Our proof-of-principle data reveal crosstalk between bacterial and neural co-cultured cells and illustrate a novel example of cross-kingdom communication between highly diverse cell types. The ability to eavesdrop on information passing between these two very different levels of biological organization will facilitate insight into evolutionary cell biology and could impact the understanding of brain-bacteria communication for diagnosis of neuronal states in health and disease.
YR 2023
FD 2023-10
LK https://hdl.handle.net/20.500.14352/130368
UL https://hdl.handle.net/20.500.14352/130368
LA eng
DS Docta Complutense
RD 17 ene 2026