RT Journal Article
T1 Light regulates the expression of the BDNF/TrkB system in the adult Zebrafish retina
A1 Sánchez Ramos, Celia
A1 Bonnin Arias, Cristina Natalia
A1 Guerrera, María Cristina
A1 García Calavia, Marta
A1 Chamorro Gutiérrez, Eva
A1 Montalbano, Giuseppe
A1 López Velasco, Salvador
A1 López Muñiz, Alfonso Joaquín
A1 Germanà, Antonino
A1 Vega Álvarez, José Antonio
AB The retina of the adult zebrafish express brain‐derived neurotrophic factor (BDNF) and its signaling receptor TrkB. This functional system is involved in the biology of the vertebrate retina and its expression is regulated by light. This study was designed to investigate the effects of cyclic (12 h light/12 h darkness) or continuous (24 h) exposure during 10 days to white light, white‐blue light, and blue light, as well as of darkness, on the expression of BDNF and TrkB in the retina. BDNF and TrkB were assessed in the retina of adult zebrafish using quantitative real‐time polymerase chain reaction and immunohistochemistry. Exposure to white, white‐blue, and blue light causes a decrease of BDNF mRNA and of BDNF immunostaining, independently of the pattern of light exposition. Conversely, in the same experimental conditions, the expression of TrkB mRNA was upregulated and TrkB immunostaining increased. Exposition to darkness diminished BDNF and TrkB mRNAs, and abolished the immunostaining for BDNF but not modified that for TrkB. These results demonstrate the regulation of BDNF and TrkB by light in the retina of adult zebrafish and might contribute to explain some aspects of the complex pathophysiology of light‐induced retinopathies.
PB Wiley
SN 1059-910X
YR 2012
FD 2012
LK https://hdl.handle.net/20.500.14352/101320
UL https://hdl.handle.net/20.500.14352/101320
LA eng
NO Sánchez-Ramos C, Bonnin-Arias C, Guerrera MC, Calavia MG, Chamorro E, Montalbano G, López-Velasco S, López-Muñiz A, Germanà A, Vega JA. Light regulates the expression of the BDNF/TrkB system in the adult zebrafish retina. Microsc Res Tech. 2013 Jan;76(1):42-9. doi: 10.1002/jemt.22133. Epub 2012 Oct 16. PMID: 23070877.
DS Docta Complutense
RD 11 abr 2025