RT Journal Article
T1 A Reliable and Standardizable Differential PCR and qPCR Methodology Assesses HER2 Gene Amplification in Gastric Cancer
A1 Juárez Martín-Delgado, Ignacio
A1 Toro Fernández, Juan Francisco
A1 Vaquero Yuste, Christian
A1 Molina Alejandre, Marta
A1 Lasa, Inmaculada
A1 Gómez, Remedios
A1 López, Adela
A1 Martín Villa, José Manuel
A1 Gutiérrez, Alberto
AB We have applied two PCR techniques, differential PCR (diffPCR) and qPCR for the identification of HER2 gene amplifications in genomic DNA of tumor and distal gastric samples from patients with gastric cancer. The diffPCR technique consists of the simultaneous amplification of the HER2 gene and a housekeeping gene by conventional PCR and the densitometric analysis of the bands obtained. We established a cut-off point based on the mean and standard deviation analyzing the DNA of 30 gastric tissues from patients undergoing non-cancer gastrectomy. diffPCR and qPCR yielded consistent results. HER2-overexpression was detected in 25% of patients and was further confirmed by immunohistochemistry and immunofluorescence. The approaches herein described may serve as complementary and reliable methods to assess HER2 amplification.
PB MDPI
SN 2079-7737
YR 2021
FD 2021-06-10
LK https://hdl.handle.net/20.500.14352/6851
UL https://hdl.handle.net/20.500.14352/6851
LA eng
NO Juárez Martín-Delgado, I., Toro Fernández, J. F., Vaquero Yuste, C. et al. «A Reliable and Standardizable Differential PCR and qPCR Methodology Assesses HER2 Gene Amplification in Gastric Cancer». Biology, vol. 10, n.o 6, junio de 2021, p. 516. DOI.org (Crossref), https://doi.org/10.3390/biology10060516.
NO Instituto de Salud Carlos III/Fondo Europeo de Desarrollo Regional
NO Universidad Complutense de Madrid/Harvard University
DS Docta Complutense
RD 26 abr 2025