RT Journal Article
T1 Proteomic analysis of synovial fibroblasts and articularchondrocytes co-cultures reveals valuable VIP-modulatedinflammatory and degradative proteins in osteoarthritis
A1 Pérez García, Selene
A1 Calamia, Valentina
A1 Hermida-Gómez, Tamara
A1 Gutiérrez-Cañas, Irene
A1 Carrión Caballo, Mar
A1 Villanueva-Romero, Raúl
A1 Castro Vázquez, David
A1 Martínez, Carmen
A1 Juarranz Moratilla, Yasmina
A1 Blanco, Francisco J.
A1 Gomáriz, Rosa P.
AB Osteoarthritis (OA) is the most common musculoskeletal disorder causing a great disability and a reduction in the quality of life. In OA, articular chondrocytes (AC) and synovial fibroblasts (SF) release innate-derived immune mediators that initiate and perpetuate inflammation, inducing cartilage extracellular matrix (ECM) degradation. Given the lack of therapies for the treatment of OA, in this study, we explore biomarkers that enable the development of new therapeutical approaches. We analyze the set of secreted proteins in AC and SF co-cultures by stable isotope labeling with amino acids (SILAC). We describe, for the first time, 115 proteins detected in SF-AC co-cultures stimulated by fibronectin fragments (Fn-fs). We also study the role of the vasoactive intestinal peptide (VIP) in this secretome, providing new proteins involved in the main events of OA, confirmed by ELISA and multiplex analyses. VIP decreases proteins involved in the inflammatory process (CHI3L1, PTX3), complement activation (C1r, C3), and cartilage ECM degradation (DCN, CTSB and MMP2), key events in the initiation and progression of OA. Our results support the anti-inflammatory and anti-catabolic properties of VIP in rheumatic diseases and provide potential new targets for OA treatment.
PB MDPI
SN 1661-6596, ESSN: 1422-0067
YR 2021
FD 2021-06-16
LK https://hdl.handle.net/20.500.14352/8694
UL https://hdl.handle.net/20.500.14352/8694
LA eng
NO Instituto de Salud Carlos III/Fondo Europeo de Desarrollo Regional (FEDER)
DS Docta Complutense
RD 30 abr 2024