RT Journal Article
T1 Topographic characterization of multispecies biofilms growing on dental implant surfaces: an in vitro model
T2 Caracterización topográfica de biopelículas multiespecies que crecen en superficies de implantes dentales: un modelo in vitro
A1 Bermejo, Patricia
A1 Sánchez Beltrán, María Del Carmen
A1 Llama Palacios, María Arantxazu
A1 Figuero Ruiz, Elena
A1 Herrera González, David
A1 Sanz Alonso, Mariano
AB Objectives: To describe the early biofilm formation over whole dental implants with its micro- and macrostructure, using an in vitro multispecies biofilm model.Material and methods: Six bacterial strains (Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans) were used to develop in vitro biofilms over whole titanium implants (growth times 12, 24, 48, 72, 96, and 120 hr). The morphology of biofilms was studied by confocal laser scanning microscopy and scanning electron microscopy, and the bacterial dynamics through quantitative polymerase chain reaction. As primary outcome variable, numbers of each species [colony-forming units per milliliter (CFU/ml)] at different incubation times were compared using the one-way analysis of variance and post hoc testing with Bonferroni's correction. Furthermore, implants were fixed in methacrylate stents to reproduce the clinical situation and biofilms were developed and analyzed by scanning electron microscopy.Results: Bacteria colonized implants in a short period of time. Biofilms reached a mature state at 96 hr, exhibiting different ratios of live/dead cells depending on their location, being the peaks of the threads the areas harboring more live bacteria. The densities of each bacteria fluctuated in time, reaching its maximum at 96 hr. Even though the coefficients of variation were high, percentages were similar to those published previously using implant surface specimens, rather than whole implants.Conclusion:c
PB Wiley
SN 0905-7161
YR 2019
FD 2019-01-17
LK https://hdl.handle.net/20.500.14352/108739
UL https://hdl.handle.net/20.500.14352/108739
LA eng
NO Bermejo P, Sánchez MC, Llama-Palacios A, Figuero E, Herrera D, Sanz M. Topographic characterization of multispecies biofilms growing on dental implant surfaces: An in vitro model. Clin Oral Implants Res. 2019 Mar;30(3):229-241. doi: 10.1111/clr.13409
DS Docta Complutense
RD 10 abr 2025