RT Journal Article
T1 Fecal Microbiota Strongly Correlates with Tissue Microbiota Composition in Colorectal Cancer but Not in Non-Small Cell Lung Cancer
A1 Vicente Valor, Juan
A1 Tesolato, Sofía Elena
A1 Paz Cabezas, Mateo
A1 Gómez Garre, María Dulce Nombre
A1 Ortega Hernández, Adriana
A1 De la Serna Esteban, Sofía Cristina
A1 Domínguez Serrano, María Inmaculada
A1 Dziakova, Jana
A1 Rivera Alonso, Daniel
A1 Jarabo Sarceda, José Ramón
A1 Gómez Martínez, Ana María
A1 Hernando Trancho, Florentino
A1 Torres García, Antonio José
A1 Iniesta Serrano, María Pilar
AB Microbiota could be of interest in the diagnosis of colorectal and non-small cell lung cancer (CRC and NSCLC). However, how the microbial components of tissues and feces reflect each other remains unknown. In this work, our main objective is to discover the degree of correlation between the composition of the tissue microbiota and that of the feces of patients affected by CRC and NSCLC. Specifically, we investigated tumor and non-tumor tissues from 38 recruited patients with CRC and 19 with NSCLC. DNA from samples was submitted for 16S rDNA metagenomic sequencing, followed by data analysis through the QIIME2 pipeline and further statistical processing with STATA IC16. Tumor and non-tumor tissue selected genera were highly correlated in both CRC and NSCLC (100% and 81.25%). Following this, we established tissue–feces correlations, using selected genera from a LEfSe analysis previously published. In CRC, we found a strong correlation between the taxa detected in feces and those from colorectal tissues. However, our data do not demonstrate this correlation in NSCLC. In conclusion, our findings strongly reinforce the utility of fecal microbiota as a non-invasive biomarker for CRC diagnosis, while highlighting critical distinctions for NSCLC. Furthermore, our data demonstrate that the microbiota components of tumor and non-tumor tissues are similar, with only minor differences being detected.
PB MDPI
SN 1661-6596
YR 2025
FD 2025-01-16
LK https://hdl.handle.net/20.500.14352/117864
UL https://hdl.handle.net/20.500.14352/117864
LA eng
NO Vicente-Valor, J.; Tesolato, S.; Paz-Cabezas, M.; Gómez-Garre, D.; Ortega-Hernández, A.; de la Serna, S.; Domínguez-Serrano, I.; Dziakova, J.; Rivera, D.; Jarabo, J.-R.; et al. Fecal Microbiota Strongly Correlates with Tissue Microbiota Composition in Colorectal Cancer but Not in Non-Small Cell Lung Cancer. Int. J. Mol. Sci. 2025, 26, 717. https:// doi.org/10.3390/ijms26020717
NO Instituto de Salud Carlos III (España)
DS Docta Complutense
RD 9 abr 2025