RT Journal Article
T1 Global scenario of the RmtE pan-aminoglycoside-resistance mechanism: emergence of the rmtE4 gene in South America associated with a hospital-related IncL plasmid
A1 Delgado Blas, José Francisco
A1 Ovejero, Cristina M.
A1 David, Sophia
A1 Serna Bernaldo, Carlos
A1 Pulido Vadillo, Mario
A1 Montero Serra, Natalia
A1 Aanensen, David M.
A1 Abadia Patiño, Lorena
A1 González Zorn, Bruno
AB Antimicrobial resistance (AMR) mechanisms, especially those conferring resistance to critically important antibiotics, are a great concern for public health. 16S rRNA methyltransferases (16S-RMTases) abolish the effectiveness of most clinically used aminoglycosides, but some of them are considered sporadic, such as RmtE. The main goals of this work were the genomic analysis of bacteria producing 16S-RMTases from a 'One Health' perspective in Venezuela, and the study of the epidemiological and evolutionary scenario of RmtE variants and their related mobile genetic elements (MGEs) worldwide. A total of 21 samples were collected in 2014 from different animal and environmental sources in the Cumaná region (Venezuela). Highly aminoglycoside-resistant Enterobacteriaceae isolates were selected, identified and screened for 16S-RMTase genes. Illumina and Nanopore whole-genome sequencing data were combined to obtain hybrid assemblies and analyse their sequence type, resistome, plasmidome and pan-genome. Genomic collections of rmtE variants and their associated MGEs were generated to perform epidemiological and phylogenetic analyses. A single 16S-RMTase, the novel RmtE4, was identified in five Klebsiella isolates from wastewater samples of Cumaná. This variant possessed three amino acid modifications with respect to RmtE1-3 (Asn152Asp, Val216Ile and Lys267Ile), representing the most genetic distant among all known and novel variants described in this work, and the second most prevalent. rmtE variants were globally spread, and their geographical distribution was determined by the associated MGEs and the carrying bacterial species. Thus, rmtE4 was found to be confined to Klebsiella isolates from South America, where it was closely related to ISVsa3 and an uncommon IncL plasmid related with hospital environments. This work uncovered the global scenario of RmtE and the existence of RmtE4, which could potentially emerge from South America. Surveillance and control measures should be developed based on these findings in order to prevent the dissemination of this AMR mechanism and preserve public health worldwide.
PB Microbiology Society
SN 2057-5858
YR 2023
FD 2023-03-24
LK https://hdl.handle.net/20.500.14352/107540
UL https://hdl.handle.net/20.500.14352/107540
LA eng
NO Delgado-Blas, J. F., Ovejero, C. M., David, S., Serna, C., Pulido-Vadillo, M., Montero, N., Aanensen, D. M., Abadia-Patiño, L., & Gonzalez-Zorn, B. (2023). Global scenario of the RmtE pan-aminoglycoside-resistance mechanism: emergence of the rmtE4 gene in South America associated with a hospital-related IncL plasmid. Microbial genomics, 9(3), mgen000946. https://doi.org/10.1099/mgen.0.000946
NO Author contributionsJ.F.D.-B., C.M.O. and B.G.-Z. were involved in the conceptualization. J.F.D.-B., C.M.O., M.P.-V. and N.M. developed the methodology. J.F.D.-B. and S.D. conducted the formal analysis. J.F.D.-B conducted the investigation. J.F.D.-B. and C.S. were involved in data curation. L.A.-P. and D.M.A. provided resources. J.F.D.-B. carried out the writing – original draft preparation. All authors participated in the writing – review and editing. J.F.D.-B. created the visualizations. B.G.-Z. was responsible for supervision and project administration.Data statement: All supporting data, code and protocols have been provided within the article or through supplementary data files. Four supplementary figures and six supplementary tables are available with the online version of this article.
NO Ministerio de Economía y Competitividad
NO Comunidad de Madrid
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 5 abr 2025