RT Journal Article
T1 Bioanalytical Detection of Steroid Abuse in Sports Based on the Androgenic Activity Measurement
A1 Martín Escudero, M. Del Pilar
A1 Muñoz Guerra, Jesus A.
A1 García Tenorio, Soledad Vargas
A1 Serrano Garde, Ester
A1 Soldevilla Navarro, Ana Belén
A1 Cortes Carrillo, Nuria
A1 Galindo Canales, Mercedes
A1 del Prado, Nayade
A1 Fuentes Ferrer, Manuel
A1 Fernández Pérez, Cristina
A1 Behnisch, Peter Alexander
A1 Brouwer, Abraham
AB The anabolic androgenic steroids (AAS) are the most frequently consumed performance enhancing drugs (PED) in sports. In the anti-doping field, the detection of AAS is carried out by the analysis of the athlete’s urine using methodologies based on liquid/gas chromatography-mass spectrometry. Unfortunately, the detection of unknown compounds is not possible. BDS’s AR CALUX® bio detection technology was studied as an indirect method to detect the administration of a single dose of testosterone (T). Twelve T and placebo single dose administered men volunteers underwent a triple-blind crossover clinical trial. The UGT2B17 deletion was present among the volunteers and evenly distributed in heterozygous (ins/del), wild-type homozygous (ins/ins), and mutated homozygous (del/del) groups. A significant statistical difference in terms of bioluminescence was observed after the testosterone (T) administration for the three types of polymorphic groups. The ratio of means between the pre- and post-T administration periods, depending on the type of polymorphism, was in group ins/ins 3.31 (CI. 95%: 2.07–5.29), group ins/del 4.15 (CI 95%: 3.05–5.67), and group del/del 2.89 (CI 95%: 2.42–3.46). The results of the study are very promising, as they may offer us the possibility of designing a detection approach that, based on intra-individual monitoring of androgenic values, in the UGT2B17 deletion type.
PB MDPI
SN 2227-9040
YR 2021
FD 2021-03-26
LK https://hdl.handle.net/20.500.14352/7394
UL https://hdl.handle.net/20.500.14352/7394
LA eng
NO The study was funded by grants awarded by the Spanish Ministry of Science and Innovation (DEP2016–78559-R; sub-program DEPO; DEP2012-40156) for competitive investigation.
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 20 jul 2024