RT Journal Article
T1 Application of Molecular Techniques to the Elucidation of the Microbial Community Structure of Antique Paintings
A1 Santos de la Sen, Antonio
A1 Cerrada, Alejandro
A1 García Fernández-Villa, Silvia
A1 San Andrés Moya, Margarita
A1 Abrusci, Concepción
A1 Marquina Díaz, Domingo
AB This paper uses molecular techniques to describe the microstructure and microbiological communities of sixteenth century artwork and their relationships. The microbiological populations, analysed by denaturing gradient gel electrophoresis (DGGE), were highly influenced by the chemical composition of the pictorial layers detected by energy-dispersive X-ray analysis. DGGE revealed that the diversity of microbial communities was lower in pictorial layers composed of pigments with metals, such as Pb, Cu and Hg, than in those found in pictorial layers without such compounds. The number of cultivable microorganisms, mainly fungi and bacteria, was very low in comparison to those found by DGGE, revealing the presence of both cultivable and as-yet-uncultivated (or not viable) species in the samples analysed. Both fungi and bacteria were present in a non-random spatial distribution. Environmental scanning electron microscopy and fluorescent in situ hybridisation analyses revealed that bacterial populations were usually found in close contact with the surface of the pictorial layers, and fungal populations were located on the bacterial biofilm. This work shows, for the first time, the correlation between the diversity of the microbial populations and the chemical composition of the pictorial layers of an artwork.
PB Springer
SN 0095-3628
SN 1432-184X
YR 2009
FD 2009-07-25
LK https://hdl.handle.net/20.500.14352/107668
UL https://hdl.handle.net/20.500.14352/107668
LA eng
NO Santos, A., Cerrada, A., García, S., San Andrés, M., Abrusci, C., & Marquina, D. (2009). Application of molecular techniques to the elucidation of the microbial community structure of antique paintings. Microbial Ecology, 58(4), 692-702. https://doi.org/10.1007/s00248-009-9564-2
NO Universidad Complutense de Madrid
NO Banco Santander
DS Docta Complutense
RD 9 abr 2025