RT Journal Article
T1 Check-control of inflammation displayed by bone marrow mesenchymal stem cells in rheumatoid arthritis patients
A1 Lamas, José Ramón
A1 Mucientes, Arkaitz
A1 Lajas Petisco, Cristina De Jesús
A1 Fernández Gutiérrez, Benjamín
A1 Lópiz Morales, María Yaiza
A1 Marco Martínez, Fernando
A1 Jover Jover, Juan Ángel
A1 Abasolo, Lydia
A1 Rodríguez Rodríguez, Luis
AB Background: Mesenchymal stem cells (MSCs) are a promising treatment of different musculoskeletal diseases including osteoarthritis and rheumatoid arthritis (RA). Results from different approaches in this treatment have been not conclusive. Aim: To analyze factors related to interactions between peripheral blood mononuclear cells (PBMCs) and MSCs and the influence of cellular activation. Materials & methods: PBMCs from RA patients and healthy controls (HC) were obtained. MSCs from bone marrow (BM-MSCs) were obtained from six donors. CD4, CD25, CD69 and CD127 expression was measured by flow cytometry. Repeated measures analysis of variance (ANOVA) models were performed using activation, co-culture with BM-MSCs and time of culture (24 h, 72 h, 6 days) as within-subject variables. Results: PBMCs activated and co-cultured with BM-MSCs showed a lower proportion of CD25-positive and CD25high/CD127low-negative cells in both RA and HC. Additionally, a maintained expression of CD69 was also observed in RA and HC when PBMCs were activated and co-cultured with BM-MSCs. Conclusion: Both PBMC activation grade and RA disease activity influence the immunomodulatory effect of BM-MSCs on T-cell activation.
PB Taylor & Francis
SN 1750-7448
YR 2019
FD 2019-05-29
LK https://hdl.handle.net/20.500.14352/107388
UL https://hdl.handle.net/20.500.14352/107388
LA eng
NO Lamas JR, Mucientes A, Lajas C, Fernández-Gutiérrez B, Lópiz Y, Marco F, Jover JA, Abásolo L, Rodriguez-Rodriguez L. Check-control of inflammation displayed by bone marrow mesenchymal stem cells in rheumatoid arthritis patients. Immunotherapy. 2019 Sep;11(13):1107-1116. doi: 10.2217/imt-2019-0091. Epub 2019 Aug 5. PMID: 31378114.
NO Ministerio de Sanidad (España)
NO Instituto de Salud Carlos III (España)
DS Docta Complutense
RD 8 sept 2024