RT Journal Article
T1 Amperometric magnetoimmunoassay for the determination of lipoprotein(a)
A1 Kaçar, Ceren
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Pedrero Muñoz, María
A1 Campuzano Ruiz, Susana
A1 Kilic, Esma
A1 Pingarrón Carrazón, José Manuel
AB A highly sensitive amperometric magnetoimmunoassay for rapid determination of lipoprotein(a) (Lp(a)), an important predictor of cardiovascular disease risk, in human serum, is described. It uses a sandwich configuration involving selective capture antibody [antiLp(a)] and biotinylated detector antibody [biotinantiLp(a)], and a streptavidin-HRP conjugate on carboxymodified magnetic beads (HOOC-MBs). The resulting MBs bearing the sandwiched immunoconjugates were captured by a magnet placed under the working electrode surface of a disposable screen-printed carbon electrode and the extent of the affinity reaction was monitored amperometrically at −0.20 V (vs a silver pseudo-reference electrode) in the presence of hydroquinone as an electron transfer mediator and upon addition of H2O2 as the enzyme substrate. The method exhibited a wide linear response range (from 0.01 to 0.5 μgmL−1), a detection limit of 4 ng mL−1, and an excellent selectivity over other serum components. The utility of the immunoassay was demonstrated by analyzing a reference serum containing a certified quantity of Lp(a). The performance of this magnetoimmunoassay compares favorably to that of an integrated amperometric immunoassay described earlier.
PB Springer
SN 0026-3672
YR 2015
FD 2015
LK https://hdl.handle.net/20.500.14352/94235
UL https://hdl.handle.net/20.500.14352/94235
LA eng
NO Kaçar, C., Torrente-Rodríguez, R.M., Pedrero, M. et al. Amperometric magnetoimmunoassay for the determination of lipoprotein(a). Microchim Acta 182, 1457–1464 (2015). https://doi.org/10.1007/s00604-015-1472-1
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 20 ene 2026