RT Journal Article
T1 Point-of-Care Diagnostic Test for Rapid Detection of Infectious Laryngotracheitis Virus by Loop-Mediated Isothermal Amplification and Nanoprobes
A1 Cea Callejo, Pablo
A1 Trenado, Claudia
A1 El Mansouri, Elías
A1 Gómez-Lucía Duato, María Esperanza
A1 Domenech Gómez, Ana María
A1 Biarnés, Mar
A1 Cuenca, J. Marco
A1 Sánchez Llatas, Christian J.
A1 Madrid González, Ricardo
A1 Benítez Rico, Laura
AB Infectious laryngotracheitis virus (ILTV), a DNA virus classified as Gallid alphaherpesvirus 1, causes a highly contagious respiratory disease in chickens, leading to significant economic losses and health risks for the poultry industry. The rapid detection of ILTV is essential to control its spread and prevent outbreaks. Traditional diagnostic methods like PCR are costly, require specialized personnel, and delay response efforts. To address this, we developed a point-of-care diagnostic test combining loop-mediated isothermal amplification (LAMP) with DNA nanoprobes on respiratory swabs. LAMP targets the ILTV-glycoprotein E (gE) gene, enabling rapid nucleic acid amplification at 65 °C without extraction, making it suitable for on-site detection. DNA nanoprobes provide a colorimetric readout visible to the naked eye. Gold nanoparticles drive this readout, as their red color, based on localized surface plasmon resonance, persists in the presence of ILTV DNA through DNA-DNA hybridization, ensuring reliable detection. The assay achieved 100% sensitivity and specificity for ILTV-gE, with a detection limit of 200 copies per reaction, allowing for the early identification of infections. The results are available within 45 min, enabling prompt measures to control ILTV spread. Cost-effective and user-friendly, this method enhances disease management and biosecurity in poultry farms.
PB MDPI
SN 1661-6596
YR 2025
FD 2025-02-25
LK https://hdl.handle.net/20.500.14352/119121
UL https://hdl.handle.net/20.500.14352/119121
LA eng
NO Cea-Callejo, P.,Trenado, C., El Mansouri, E., Gomez-Lucia, E., Doménech, A., Biarnés, M., Cuenca, J.M., Sánchez-Llatas, C.J., Madrid, R., Benítez,(2025) L. Point-of-Care Diagnostic Test for Rapid Detection of Infectious Laryngotracheitis Virus by Loop-Mediated Isothermal Amplification and Nanoprobes. Int. J. Mol. Sci., 26, 1971. https://doi.org/10.3390/ijms26051971
NO Author Contributions:Conceptualization, P.C.-C., R.M. and L.B.; formal analysis, P.C.-C., C.T. and R.M.; investigation, P.C.-C., C.T., E.E.M., M.B., J.M.C. and C.J.S.-L.; methodology P.C.-C., R.M. and L.B.; writing—original draft, P.C.-C., A.D., R.M. and L.B.; writing—review and editing, P.C.-C., C.T., E.G.-L., A.D., R.M. and L.B.; resources, E.G.-L., A.D., M.B. and J.M.C.; funding and acquisition, A.D. and L.B.; supervision, A.D., R.M. and L.B.; project administration, R.M. and L.B. All authors have read and agreed to the published version of the manuscript.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 5 abr 2025