RT Journal Article
T1 Antigen-functionalized turnip mosaic virus nanoparticles increase antibody sensing in saliva. A case study with SARS-CoV-2 RBD
A1 Truchado Martín, Daniel Alejandro
A1 Medrano Arranz, Carlos
A1 Rincón, Sara
A1 Zurita, Lucía
A1 Ponz, Fernando
AB Nanoparticles derived from plant viruses play an important role in nanomedicine due to their biocompatibility, self-assembly and easily-modifiable surface. In this study, we developed a novel platform for increasing antibody sensing using viral nanoparticles derived from turnip mosaic virus (TuMV) functionalized with SARS-CoV-2 receptor binding domain (RBD) through three different methods: chemical conjugation, gene fusion and the SpyTag/SpyCatcher technology. Even though gene fusion turned out to be unsuccessful, the other two constructs were proven to significantly increase antibody sensing when tested with saliva of patients with different infection and vaccination status to SARS-CoV-2. Our findings show the high potential of TuMV nanoparticles in the fields of diagnostics and immunodetection, being especially attractive for the development of novel antibody sensing devices.
PB Elsevier
SN 0732-8893
YR 2024
FD 2024-04-07
LK https://hdl.handle.net/20.500.14352/103201
UL https://hdl.handle.net/20.500.14352/103201
LA eng
NO Ministerio de Universidades (España)
NO Comunidad de Madrid
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 6 abr 2025