RT Journal Article
T1 Enhancement of in vivo supraspinatus tendon–to-bone healing with an alginate-chitin scaffold and rhBMP-2
A1 Camila, Arvinius
A1 Civantos, Ana
A1 Rodríguez Bobada, Cruz
A1 Rojo, Francisco Javier
A1 Pérez Gallego, Daniel
A1 Lópiz Morales, María Yaiza
A1 Marco Martínez, Fernando
AB Introduction: Rotator cuff disorders present a high retear rate despite advances in surgical treatment. Tissue engineering could therefore be interesting in order to try to enhance a more biological repair. RhBMP-2 is one of the most osteogenic growth factors and it also induces the formation of collagen type I. However, it has a short half-life and in order to get a more stable release over time it could be integrated in a more slowly degradable carrier, such as an alginate-chitin scaffold. The aim of this study was to investigate the role of the alginate-chitin scaffold alone and in combination with different concentrations of rhBMP-2 when applied on chronic rotator cuff lesions in a rat model.Materials and methods: We performed an experimental study with 80 Sprague-Dawley rats, 8 months old, with a chronic rupture of the supraspinatus tendon that was repaired with a modified Mason Allen suture. A scaffold was applied over the suture and 4 groups were obtained; suture (S) only suture, double control (DC) alginate and chitin scaffold, single sample (SS) scaffold of alginate with rhBMP-2 (20 µg rhBMP-2) and chitin, double sample (DS) a scaffold containing alginate with rhBMP-2 and chitin with rhBMP-2 (40 µg rhBMP-2). Macroscopic, histological and biomechanical studies were performed at 4 months after reparation.Results: The modified Åström and Rausing's histological scale (the higher the score the worse outcome, 0 points=native tendon) was applied: S got 52 points compared to DC 30 (p = 0,034), SS 22 (p = 0,009) and DS 16 (p = 0,010). Biomechanically the maximum load was highest in DC (63,05 N), followed by DS (61,60 N), SS (52,35 N) and S (51,08), p = 0,025 DS vs S. As to the elastic constant a higher value was obtained in DC (16,65), DS (12,55) and SS (12,20) compared to S (9,33), p = 0,009 DC vs S and 0,034 DS vs S.Conclusions: The alginate-chitin scaffold seems to promote a more biological response after the reparation of a chronic rotator cuff lesion. Its effect is further enhanced by the addition of rhBMP-2 since the osteotendinous junction is more native-like and has better biomechanical properties.
PB Elsevier
SN 1879-0267
YR 2020
FD 2020-11-09
LK https://hdl.handle.net/20.500.14352/107403
UL https://hdl.handle.net/20.500.14352/107403
LA eng
NO Arvinius C, Civantos A, Rodríguez-Bobada C, Rojo FJ, Pérez-Gallego D, Lopiz Y, Marco F. Enhancement of in vivo supraspinatus tendon-to-bone healing with an alginate-chitin scaffold and rhBMP-2. Injury. 2021 Jan;52(1):78-84. doi: 10.1016/j.injury.2020.11.019. Epub 2020 Nov 9. PMID: 33223258.
DS Docta Complutense
RD 11 oct 2024