RT Journal Article
T1 D-Root: a system for cultivating plants with the roots indarkness or under different light conditions
A1 Silva Navas, Javier
A1 Moreno Risueno, Miguel A.
A1 Manzano, Concepción
A1 Pallero Baena, Mercedes
A1 Navarro Neila, Sara
A1 Téllez Robledo, Bárbara
A1 García Mina, José M.
A1 Baigorri, Roberto
A1 Gallego Rodríguez, Francisco Javier
A1 Pozo, Juan C. del
AB In nature roots grow in the dark and away from light (negative phototropism). However, most currentresearch in root biology has been carried out with the root system grown in the presence of light. Here, wehave engineered a device, called Dark-Root (D-Root), to grow plants in vitro with the aerial part exposed tothe normal light/dark photoperiod while the roots are in the dark or exposed to specific wavelengths orlight intensities. D-Root provides an efficient system for cultivating a large number of seedlings and easilycharacterizing root architecture in the dark. At the morphological level, root illumination shortens rootlength and promotes early emergence of lateral roots, therefore inducing expansion of the root system. Sur-prisingly, root illumination also affects shoot development, including flowering time. Our analyses alsoshow that root illumination alters the proper response to hormones or abiotic stress (e.g. salt or osmoticstress) and nutrient starvation, enhancing inhibition of root growth. In conclusion, D-Root provides a grow-ing system closer to the natural one for assaying Arabidopsis plants, and therefore its use will contribute toa better understanding of the mechanisms involved in root development, hormonal signaling and stressresponses.
PB Wiley
SN 0960-7412
YR 2015
FD 2015-08-27
LK https://hdl.handle.net/20.500.14352/23372
UL https://hdl.handle.net/20.500.14352/23372
LA eng
NO Unión Europea. FP7
NO Ministerio de Economía y Competitividad (MINECO)
NO MINECO and FEDER
DS Docta Complutense
RD 7 may 2024