RT Journal Article
T1 Ipriflavone-loaded mesoporous nanospheres with potential applications for periodontal treatment.
A1 Casarrubios Molina, Laura
A1 Gómez Cerezo, María Natividad
A1 Feito Castellano, María José
A1 Vallet Regí, María Dulce Nombre
A1 Arcos Navarrete, Daniel
A1 Portolés Pérez, María Teresa
AB The incorporation and effects of hollow mesoporous nanospheres in the systemSiO2–CaO (nanoMBGs) containing ipriflavone (IP), a synthetic isoflavone that prevents osteoporosis, were evaluated. Due to their superior porosity and capability to host drugs, these nanoparticles are designed as a potential alternative to conventional bioactive glasses for the treatment of periodontal defects. To identify the endocytic mechanisms by which these nanospheres are incorporated within the MC3T3-E1 cells, five inhibitors (cytochalasin B, cytochalasin D, chlorpromazine, genistein and wortmannin) were used before the addition of these nanoparticles labeled with fluorescein isothiocyanate (FITC–nanoMBGs). The results indicate that nanoMBGs enter the pre-osteoblasts mainly through clathrin-dependent mechanisms and in a lower proportion by macropinocytosis. The present study evidences the active incorporation of nanoMBG–IPs by MC3T3-E1 osteoprogenitor cells that stimulate their differentiation into mature osteoblast phenotype with increased alkaline phosphatase activity. The final aim of this study is to demonstrate the biocompatibility and osteogenic behavior of IP-loaded bioactive nanoparticles to be used for periodontal augmentation purposes and to shed light on internalization mechanisms that determine the incorporation of these nanoparticles into the cells.
PB MDPI
SN 2079-4991
YR 2020
FD 2020-12-21
LK https://hdl.handle.net/20.500.14352/7650
UL https://hdl.handle.net/20.500.14352/7650
LA eng
NO RESEARCHER ID L-6167-2014 (Daniel Arcos Navarrete)ORCID 0000-0002-5367-7272 (Daniel Arcos Navarrete)RESEARCHER ID M-3378-2014 (María Vallet Regí)ORCID 0000-0002-6104-4889 (María Vallet Regí)RESEARCHER ID U-1678-2017 (María Teresa Portolés Pérez)ORCID 0000-0002-9681-0184 (María Teresa Portolés Pérez)
NO Unión Europea. Horizonte 2020
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 28 mar 2025