RT Journal Article
T1 Supramolecular Enzymatic Labeling for Aptamer Switch-Based Electrochemical Biosensor
A1 Villalonga Chico, Anabel
A1 Parrado Quintela, Concepción
A1 Diaz, Raúl
A1 Sánchez Sánchez, Alfredo
A1 Mayol Hornero, Beatriz
A1 Martínez-Ruíz, Paloma
A1 Vilela García, Diana
A1 Villalonga Santana, Reynaldo
AB Here we report a novel labeling strategy for electrochemical aptasensors based on enzymatic marking via supramolecular host–guest interactions. This approach relies on the use of an adamantane-modified target-responsive hairpin DNA aptamer as an affinity bioreceptor, and a neoglycoconjugate of β-cyclodextin (CD) covalently attached to a redox enzyme as a labeling element. As a proof of concept, an amperometric aptasensor for a carcinoembryonic antigen was assembled on screen-printed carbon electrodes modified with electrodeposited fern-like gold nanoparticles/graphene oxide and, by using a horseradish peroxidase-CD neoglycoenzyme as a biocatalytic redox label. This aptasensor was able to detect the biomarker in the concentration range from 10 pg/mL to 1 ng/mL with a high selectivity and a low detection limit of 3.1 pg/mL in human serum samples.
PB MDPI
SN 2079-6374
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/72223
UL https://hdl.handle.net/20.500.14352/72223
LA eng
NO Ministerio de Economía y Competitividad
NO MICINN
DS Docta Complutense
RD 6 abr 2025