RT Journal Article
T1 CD69 Modulates Sphingosine-1-Phosphate-Induced Migration of Skin Dendritic Cells
A1 Lamana Domínguez, Amalia
A1 Martin, Pilar
A1 Fuente, Hortensia de la
A1 Martinez-Muñoz, Laura
A1 Cruz Adalia, Aranzazu
A1 Ramirez-Huesca, Marta
A1 Escribano, Cristina
A1 Gollmer, Kathrin
A1 Mellado, Mario
A1 Stein, Jens
A1 Rodriguez-Fernandez, Jose Luis
A1 Sanchez-Madrid, Francisco
A1 Martinez del Hoyo, Gloria
AB In this study, we have investigated the role of CD69, an early inducible leukocyte activation receptor, in murine dendritic cell (DC) differentiation, maturation, and migration. Skin DCs and DC subsets present in mouse lymphoid organs express CD69 in response to maturation stimuli. Using a contact sensitization model, we show that skin DCs migrated more efficiently to draining lymph nodes (LNs) in the absence of CD69. This was confirmed by subcutaneous transfer of CD69–/– DCs, which presented an increased migration to peripheral LNs. Two-photon microscopy analysis showed that once DCs reached the LNs, CD69 deficiency did not alter DC interstitial motility in the LNs. Chemotaxis to sphingosine-1-phosphate (S1P) was enhanced in CD69–/– DCs compared with wild-type DCs. Accordingly, we detected a higher expression of S1P receptor type-1 (S1P1) by CD69–/– DCs, whereas S1P3 expression levels were similar in wild-type and CD69–/– DCs. Moreover, in vivo treatment with S1P analogs SEW2871 and FTY720 during skin sensitization reduced skin DC migration to peripheral LNs. These results suggest that CD69 regulates S1P-induced skin DC migration by modulating S1P1 function. Together, our findings increase our knowledge on DC trafficking patterns in the skin, enabling the development of new directed therapies using DCs for antigen (Ag) delivery.
PB Elsevier
SN 0022-202X
YR 2011
FD 2011
LK https://hdl.handle.net/20.500.14352/94309
UL https://hdl.handle.net/20.500.14352/94309
LA eng
NO Lamana, Amalia, et al. «CD69 Modulates Sphingosine-1-Phosphate-Induced Migration of Skin Dendritic Cells». Journal of Investigative Dermatology, vol. 131, n.o 7, julio de 2011, pp. 1503-12. https://doi.org/10.1038/jid.2011.54.
NO This work was supported by grants MEICA from Genoma España Foundation, SAF 2008-02635 from Ministerio de Ciencia e Innovación, INSINET from Comunidad Autónoma de Madrid, FONCICYT-95395, ALA/127249, and RECAVA RD06/0014-0030 from Instituto de Salud Carlos III to FSM and SAF2008-02719 from Ministerio de Ciencia e Innovación to PM. GMH is supported by Instituto de Salud Carlos III. CNIC is supported by the Spanish Ministry of Health and Consumer Affairs and the Pro-CNIC Foundation. We thank Simon Bartlett for editing the manuscript.
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Comunidad de Madrid
NO Instituto de Salud Carlos III
NO Consejo Nacional de Humanidades, Ciencias y Tecnologías (México)
NO Ministerio de Sanidad y Consumo (España)
DS Docta Complutense
RD 21 abr 2025