RT Journal Article
T1 Heterologous Expression of the Phytochelatin Synthase CaPCS2 from Chlamydomonas acidophila and Its Effect on Different Stress Factors in Escherichia coli
A1 Díaz Del Toro, Silvia
A1 Aguilera, Ángeles
A1 González de Figueras, Carolina
A1 De Francisco Martínez, Patricia
A1 Olsson, Sanna
A1 Puente-Sánchez, Fernando
A1 González-Pastor, José Eduardo
AB Phytochelatins (PCs) are cysteine-rich small peptides, enzymatically synthesized from reduced glutathione (GSH) by cytosolic enzyme phytochelatin synthase (PCS). The open reading frame (ORF) of the phytochelatin synthase CaPCS2 gene from the microalgae Chlamydomonas acidophila was heterologously expressed in Escherichia coli strain DH5α, to analyze its role in protection against various abiotic agents that cause cellular stress. The transformed E. coli strain showed increased tolerance to exposure to different heavy metals (HMs) and arsenic (As), as well as to acidic pH and exposure to UVB, salt, or perchlorate. In addition to metal detoxification activity, new functions have also been reported for PCS and PCs. According to the results obtained in this work, the heterologous expression of CaPCS2 in E. coli provides protection against oxidative stress produced by metals and exposure to different ROS-inducing agents. However, the function of this PCS is not related to HM bioaccumulation.
PB MDPI
YR 2022
FD 2022-06-23
LK https://hdl.handle.net/20.500.14352/103047
UL https://hdl.handle.net/20.500.14352/103047
LA eng
NO Ministerio de Ciencia e Innovación (MCIN)/Agencia estatal de Investigación
NO Fondo Europeo de Desarrollo Regional  (FEDER)
DS Docta Complutense
RD 24 ago 2024