RT Journal Article T1 A Novel Niosome-Encapsulated Essential Oil Formulation to Prevent Aspergillus flavus Growth and Aflatoxin Contamination of Maize Grains During Storage A1 García-Díaz, Marta A1 Patiño Álvarez, Aurora Belén A1 Vázquez Estévez, María Covadonga Inmaculada A1 Gil Serna, Jessica AB Aflatoxin (AF) contamination of maize is a major concern for food safety. The use of chemical fungicides is controversial, and it is necessary to develop new effective methods to control Aspergillus flavus growth and, therefore, to avoid the presence of AFs in grains. In this work, we tested in vitro the effect of six essential oils (EOs) extracted from aromatic plants. We selected those from Satureja montana and Origanum virens because they show high levels of antifungal and antitoxigenic activity at low concentrations against A. flavus. EOs are highly volatile compounds and we have developed a new niosome-based encapsulation method to extend their shelf life and activity. These new formulations have been successfully applied to reduce fungal growth and AF accumulation in maize grains in a small-scale test, as well as placing the maize into polypropylene woven bags to simulate common storage conditions. In this latter case, the antifungal properties lasted up to 75 days after the first application PB MDPI SN 2072-6651 YR 2019 FD 2019-11-06 LK https://hdl.handle.net/20.500.14352/6367 UL https://hdl.handle.net/20.500.14352/6367 LA eng NO Ministerio de Ciencia, Innovación y Universidades (MICINN) DS Docta Complutense RD 10 abr 2025