RT Journal Article
T1 Chalcogenides-based Tubular Micromotors in Fluorescent Assays
A1 Asunción-Nadal, Víctor de la
A1 Pacheco Jérez, Marta
A1 Jurado-Sánchez, Beatriz
A1 Escarpa, Alberto
AB WS2/Pt and MoS2/Pt bubble propelled micromotors are used as “on-the-fly” sensing platforms in bioassays, using a highly selective affinity peptide probe for “OFF-ON” detection of Escherichia coli as a model endotoxin. The different outer micromotor surface characteristics play an important role in the sensing performance. The relatively high outer surface of WS2/Pt micromotors results in a 3.5-fold increase in sensitivity compared to MoS2/Pt micromotors due to enhanced peptide probe loading and release. The peptide-modified WS2 micromotors are used as a low-cost and high-throughput approach for the determination of E. coli endotoxin after only 60 s, with a limit of detection of 1.9 ng mL–1 and high selectivity. The method has been validated using spiked samples (tap water, blood serum, and saliva) and bacteria media (blank broth, Staphylococcus aureus, and E. coli). The concept can be extended to the analysis of other (bio)-analytes and easily incorporated into portable instrumentation, holding great promise in a myriad of clinical, environmental, or food-safety applications.
PB American Chemical Society
SN 0003-2700
YR 2020
FD 2020-06-11
LK https://hdl.handle.net/20.500.14352/96081
UL https://hdl.handle.net/20.500.14352/96081
LA eng
NO V. Asunción-Nadal, M. Pacheco, B. Jurado-Sánchez*, A. Escarpa*. Chalcogenides-based tubular micromotors in fluorescent assays. Anal. Chem. 2020, 92, 9188-9193. IF: 6,986; Rank (Chemistry, Analytical): 6/94 (Q1).
NO University of Alcalá
NO Spanish Ministry of Science, Innovation and Universities
NO Spanish Ministry of Economy, Industry and Competitiveness
NO Community of Madrid
NO TRANSNANOAVANSENS program
DS Docta Complutense
RD 21 abr 2025