%0 Journal Article
%A Bermejo, Patricia
%A Sánchez Beltrán, María Del Carmen
%A Llama Palacios, María Arantxazu
%A Figuero Ruiz, Elena
%A Herrera González, David
%A Sanz Alonso, Mariano
%T Biofilm formation on dental implants with different surface micro-topography: An in vitro study
%D 2019
%@ 0905-7161
%U https://hdl.handle.net/20.500.14352/94527
%X Objectives: To   compare biofilm formation on   whole     dental     titanium implants with different surface micro‐topography.Methods: A  multispecies in  vitro    biofilm model    consisting of  initial    (Streptococcus ora‐lis  and  Actinomyces  naeslundii),  early    (Veillonella  parvula),  secondary (Fusobacterium nucleatum)  and   late   colonizers (Porphyromonas  gingivalis  and  Aggregatibacter  actino‐mycetemcomitans)  was   grown    for   96  hr  on  sterile    titanium dental    implants with   either minimal (Sa:  0.5–1.0 mm)   or  moderate‐roughness titanium surfaces (Sa:  1.1–2.0 mm). The    resulting biofilms were    studied with    Confocal Laser    Scanning Microscopy (CLSM) and   Scanning Electron Microscope. Concentrations (colony‐forming units    per mL   [CFU/ml]) of  each    bacterium were    measured by  quantitative Polymerase Chain Reaction (qPCR) and compared by Student t tests.Results: A  biofilm, located mainly     at  the   peak    and   lateral    areas    of  the   implant threads, was   observed on  both   implant surfaces, with   a  greater biomass and   a  greater live/dead ratio    in  moderate‐ compared to  minimal‐roughness surface implants. Statistically sig ‐nificant higher     values     of  total    bacteria (mean    difference =  2.61    ×  107 CFU/ml; 95% confidence interval —  CI  [1.91    ×  106;  5.02    ×  107]; p  =  0.036), F.    Nucleatum  (mean  difference =  4.43    ×  106 CFU/ml; 95%   CI  [1.06   ×  106;  7.80    ×  106]; p  =  0.013) and A.    actinomycetemcomitans (mean    difference =  2.55   ×  107 CFU/ml; 95%  CI  [1.07   ×  107; 4.04   ×  107]; p  =  0.002), were    found    in  the   moderate‐ compared to  minimal‐roughness surface dental implants.Conclusions: Implants with    moderate‐roughness surfaces accumulated more    bacte  ‐rial   biomass and   significant higher     number of  pathogenic bacteria (F. nucleatum and A.    actinomycetemcomitans), when    compared to  implants with   minimal‐roughness sur ‐faces, within a similar biofilm structure.
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