%0 Journal Article
%A Méndez González, Diego
%A Silva Ibáñez, Pedro Pablo
%A Valiente Dies, Fernando
%A Gómez Calderón, Óscar
%A Méndez González, Juan Luis
%A Laurenti, Marco
%A Egatz-Gómez, Ana
%A Díaz García, Elena
%A Rubio Retama, Benito Jorge
%A Melle Hernández, Sonia
%T Oligonucleotide sensor based on magnetic capture and photoligation of upconverting nanoparticles in solid surfaces
%D 2021
%@ 0021-9797
%U https://hdl.handle.net/20.500.14352/7942
%X In this work, we present a luminescence platform that can be used as point of care system for determining the presence and concentration of specific oligonucleotide sequences. This sensor exhibited a limit of detection as low as 50 fM by means of: i) the use of single-stranded DNA (ssDNA) functionalized magnetic microparticles that captured and concentrated ssDNA-upconverting nanoparticles (ssDNA-UCNPs) on a solid support, when the target sequence (miR-21-5p DNA-analogue) was in the sample, and ii) a photoligation reaction that covalently linked the ssDNA-UCNPs and the ssDNA magnetic microparticles, allowing stringent washes. The presented sensor showed a similar limit of detection when the assays were conducted in samples containing total miRNA extracted from human serum, demonstrating its suitability for detecting small specific oligonucleotide sequences under real-like conditions. The strategy of combining UCNPs, magnetic microparticles, and photoligation reaction provides new insight into low-cost, rapid, and ultra-sensitive detection of oligonucleotide sequences.
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