RT Journal Article
T1 Oligonucleotide sensor based on magnetic capture and photoligation of upconverting nanoparticles in solid surfaces
A1 Méndez González, Diego
A1 Silva Ibáñez, Pedro Pablo
A1 Valiente Dies, Fernando
A1 Gómez Calderón, Óscar
A1 Méndez González, Juan Luis
A1 Laurenti, Marco
A1 Egatz-Gómez, Ana
A1 Díaz García, Elena
A1 Rubio Retama, Benito Jorge
A1 Melle Hernández, Sonia
AB In this work, we present a luminescence platform that can be used as point of care system for determining the presence and concentration of specific oligonucleotide sequences. This sensor exhibited a limit of detection as low as 50 fM by means of: i) the use of single-stranded DNA (ssDNA) functionalized magnetic microparticles that captured and concentrated ssDNA-upconverting nanoparticles (ssDNA-UCNPs) on a solid support, when the target sequence (miR-21-5p DNA-analogue) was in the sample, and ii) a photoligation reaction that covalently linked the ssDNA-UCNPs and the ssDNA magnetic microparticles, allowing stringent washes. The presented sensor showed a similar limit of detection when the assays were conducted in samples containing total miRNA extracted from human serum, demonstrating its suitability for detecting small specific oligonucleotide sequences under real-like conditions. The strategy of combining UCNPs, magnetic microparticles, and photoligation reaction provides new insight into low-cost, rapid, and ultra-sensitive detection of oligonucleotide sequences.
PB Elsevier
SN 0021-9797
YR 2021
FD 2021-03-11
LK https://hdl.handle.net/20.500.14352/7942
UL https://hdl.handle.net/20.500.14352/7942
LA eng
NO Méndez González, D., Silva Ibáñez, P. P., Valiente Dies, F. et al. «Oligonucleotide Sensor Based on Magnetic Capture and Photoligation of Upconverting Nanoparticles in Solid Surfaces». Journal of Colloid and Interface Science, vol. 596, agosto de 2021, pp. 64-74. DOI.org (Crossref), https://doi.org/10.1016/j.jcis.2021.02.093.
NO Ministerio de Economía, Comercio y Empresa (España)
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Comunidad de Madrid/Fondo Europeo de Desarrollo Regional
NO Universidad Complutense de Madrid/Banco de Santander
NO Comisión Nacional de Investigación Científica y Tecnológica de España
NO European Commission for a YEI program contract
DS Docta Complutense
RD 17 abr 2025