RT Journal Article
T1 Tunable degrees of neurodegeneration in rats based on microsphere‑induced models of chronic glaucoma
T2 Grados ajustables de neurodegeneración en ratas basados ​​en modelos de glaucoma crónico inducidos por microesferas
A1 Bravo Osuna, Irene
A1 Aragón Navas, Alba
A1 García Herranz, David
A1 Herrero Vanrell, María Del Rocío
A1 Rodrigo María, Jesús
A1 Subias, Manuel
A1 Montolío, Alberto
A1 Cegoñino, José
A1 Martínez-Rincón, Teresa
A1 Méndez-Martínez, Silvia
A1 Pablo Luis, Emilio
A1 Pérez del Palomar, Amaya
A1 García-Martín, Elena
AB This study compares four different animal models of chronic glaucoma against normal aging over 6 months. Chronic glaucoma was induced in 138 Long–Evans rats and compared against 43 agedmatched healthy rats. Twenty‑five rats received episcleral vein sclerosis injections (EPIm cohort) while the rest were injected in the eye anterior chamber with a suspension of biodegradable microspheres: 25 rats received non‑loaded microspheres (N‑L Ms cohort), 45 rats received microspheres loaded with dexamethasone (MsDexa cohort), and 43 rats received microspheres co‑loaded with dexamethasone and fibronectin (MsDexaFibro cohort). Intraocular pressure, neuroretinal function, structure and vitreous interface were evaluated. Each model caused different trends in intraocular pressure, produced specific retinal damage and vitreous signals. The steepest and strongest increase in intraocular pressure was seen in the EPIm cohort and microspheres models were more progressive. The EPIm cohort presented the highest vitreous intensity and percentage loss in the ganglion cell layer, the MsDexa cohort presented the greatest loss in the retinal nerve fiber layer, and the MsDexaFibro cohort presented the greatest loss in total retinal thickness. Function decreased differently among cohorts. Using biodegradable microspheres models it is possible to generate tuned neurodegeneration. These results support the multifactorial nature of glaucoma based on several noxa.
PB Nature Research
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/118045
UL https://hdl.handle.net/20.500.14352/118045
LA eng
NO Rodrigo MJ, Bravo-Osuna I, Subias M, Montolío A, Cegoñino J, Martinez-Rincón T, Mendez-Martinez S, Aragón-Navas A, Garcia-Herranz D, Pablo LE, Herrero-Vanrell R, Del Palomar AP, Garcia-Martin E. Tunable degrees of neurodegeneration in rats based on microsphere-induced models of chronic glaucoma. Sci Rep 2022;12:20622. https://doi.org/10.1038/s41598-022-24954-4.
NO Universidad Complutense de Madrid (España)
NO Instituto de Salud Carlos III (España)
NO European Social Fund
NO European Regional Development Fund
DS Docta Complutense
RD 4 abr 2025