RT Journal Article
T1 Daily Lipolysis Gene Expression in Male Rat Mesenteric Adipose Tissue: Obesity and Melatonin Effects
A1 Cano Barquilla, María Pilar
A1 Jiménez Ortega, Vanesa
A1 Fernández Mateos, María Del Pilar
A1 Virto Ruiz, Leire
A1 Maldonado Bautista, Estela
A1 Pérez De Miguelsanz, María Juliana
A1 Esquifino, Ana I.
AB Melatonin is involved in various functions such as the timing of circadian rhythms, energy metabolism, and body mass gain in experimental animals. However, its effects on adipose tissue lipid metabolism are still unclear. This study analyzes the effects of melatonin on the relative gene expression of lipolytic proteins in rat mesenteric adipose tissue and free fatty acid (FFA) and glycerol plasma levels of male Wistar rats fed a high-fat (HFD) or maintenance diet. Four experimental groups were established: control, obese, and control or obese plus 2.3 mg/kg/day of melatonin in tap water. After 11 weeks, animals were sacrificed at different times throughout a 24 h cycle, and mesenteric adipose tissue and plasma samples were collected and analyzed. Cgi58, Perilipin, and Dgat1 gene expression, as well as FFA and glycerol concentrations, showed rhythm patterns in the control group. HFD disrupted those rhythm patterns and increased FFA and glycerol concentrations during the dark photoperiod. In both melatonin-treated groups, almost all analyzed genes showed circadian patterns. Notably, melatonin significantly prevented the increase in FFA levels during the dark photoperiod in obese rats (obese group: ~1100 mM vs. obese + melatonin group: ~600 μM, similar to control levels). However, the rhythmic pattern observed in control animals was not sustained. According to our results, melatonin could regulate circadian gene transcription of mesenteric adipose tissue lipolysis proteins. The effect of melatonin on preventing elevated FFA plasma levels associated with high-fat diet intake warrants further investigation.
PB PubMed
YR 2025
FD 2025-01
LK https://hdl.handle.net/20.500.14352/118344
UL https://hdl.handle.net/20.500.14352/118344
LA eng
NO Cano-Barquilla P, Jiménez-Ortega V, Fernández-Mateos P, Virto L, Maldonado Bautista E, Perez-Miguelsanz J, Esquifino AI. Daily Lipolysis Gene Expression in Male Rat Mesenteric Adipose Tissue: Obesity and Melatonin Effects. Int J Mol Sci. 2025 Jan 11;26(2):577. doi: 10.3390/ijms26020577. PMID: 39859293; PMCID: PMC11765279.
NO Ministerio de Educación, Formación Profesional y Deportes
NO Universidad Complutense de Madrid
DS Docta Complutense
RD 11 abr 2025