%0 Journal Article
%A Merino Serrais, Paula
%A Plaza Alonso, Sergio
%A Hellal, Farida
%A Valero Freitag, Susana
%A Kastanauskaite, Asta
%A Muñoz Céspedes, Alberto
%A Plesnila, Nikolaus
%A Felipe, Javier de
%T Microanatomical study of pyramidal neurons in the contralesional somatosensory cortex after experimental ischemic stroke
%D 2022
%@ 1047-3211, ESSN: 1460-2199
%U https://hdl.handle.net/20.500.14352/72133
%X At present, many studies support the notion that after stroke, remote regions connected to the infarcted area are also affected and may contribute to functional outcome. In the present study, we have analyzed possible microanatomical alterations in pyramidal neurons from the contralesional hemisphere after induced stroke. We performed intracellular injections of Lucifer yellow in pyramidal neurons from layer III in the somatosensory cortex of the contralesional hemisphere in an ischemic stroke mouse model. A detailed 3-dimensional analysis of the neuronal complexity and morphological alterations of dendritic spines was then performed. Our results demonstrate that pyramidal neurons from layer III in the somatosensory cortex of the contralesional hemisphere show selective changes in their dendritic arbors, namely, less dendritic complexity of the apical dendritic arbor—but no changes in the basal dendritic arbor. In addition, we found differences in spine morphology in both apical and basal dendrites comparing the contralesional hemisphere with the lesional hemisphere. Our results show that pyramidal neurons of remote areas connected to the infarct zone exhibit a series of selective changes in neuronal complexity and morphological distribution of dendritic spines, supporting the hypothesis that remote regions connected to the peri-infarcted area are also affected after stroke.
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