RT Journal Article
T1 Microanatomical study of pyramidal neurons in the contralesional somatosensory cortex after experimental ischemic stroke
A1 Merino Serrais, Paula
A1 Plaza Alonso, Sergio
A1 Hellal, Farida
A1 Valero Freitag, Susana
A1 Kastanauskaite, Asta
A1 Muñoz Céspedes, Alberto
A1 Plesnila, Nikolaus
A1 Felipe, Javier de
AB At present, many studies support the notion that after stroke, remote regions connected to the infarcted area are also affected and may contribute to functional outcome. In the present study, we have analyzed possible microanatomical alterations in pyramidal neurons from the contralesional hemisphere after induced stroke. We performed intracellular injections of Lucifer yellow in pyramidal neurons from layer III in the somatosensory cortex of the contralesional hemisphere in an ischemic stroke mouse model. A detailed 3-dimensional analysis of the neuronal complexity and morphological alterations of dendritic spines was then performed. Our results demonstrate that pyramidal neurons from layer III in the somatosensory cortex of the contralesional hemisphere show selective changes in their dendritic arbors, namely, less dendritic complexity of the apical dendritic arbor—but no changes in the basal dendritic arbor. In addition, we found differences in spine morphology in both apical and basal dendrites comparing the contralesional hemisphere with the lesional hemisphere. Our results show that pyramidal neurons of remote areas connected to the infarct zone exhibit a series of selective changes in neuronal complexity and morphological distribution of dendritic spines, supporting the hypothesis that remote regions connected to the peri-infarcted area are also affected after stroke.
PB Oxford Open
SN 1047-3211, ESSN: 1460-2199
YR 2022
FD 2022-03-30
LK https://hdl.handle.net/20.500.14352/72133
UL https://hdl.handle.net/20.500.14352/72133
LA eng
NO Ministerio de Ciencia e Innovación (MICINN)
NO Network of European Funding for Neuroscience Research
NO Interdisciplinary Platform Cajal Blue Brain (CSIC,Spain)
NO Centro de Investigación Biomédica en Red sobre Enfermedades Neurodegenerativas
NO Deutsche Forschungsge-meinschaft (DFG,German Research Foundation)
DS Docta Complutense
RD 8 may 2024