RT Journal Article T1 Chromatic evolution, chemical changes, and biological colonisation in the quarry fronts of the Santullán limestone massif (Cantabria, Spain): implication for the mitigation of visual impact in mountain quarrying A1 Iglesias-Martínez, Mario A1 Fernández-Suarez, Jorge A1 Ríos, Asunción de los A1 Xabier Arroyo, A1 García Lorenzo, María de la Luz A1 Crespo Feo, María Elena A1 Plet, Chloe A1 Andrés, Pedro de AB The visual impact of the chromatic contrast between quarry faces and rocky outcrops represents one of the prominent disturbances to natural environments. This study, therefore, aims to quantify color changes over time in quarries by analyzing three faces of an active limestone quarry in Santullán, Cantabria, that were exposed to atmospheric conditions in 1978, 2003 and 2021. To achieve this, the contribution of biological colonisation to natural darkening, along with the physicochemical changes occurring on the quarry faces, have been evaluated using scanning electron microscopy in secondary and backscattered electron mode, UV–Vis spectrophotometric techniques, Raman spectroscopy and XRD and XRF analysis. The analysis revealed that the color change was primarily due to microbial colonisation rather than oxidative chemical reactions. Although color change does not follow a direct and progressive relationship with exposure time, biological colonisation, identified primarily as microbial communities dominated by phototrophic microorganisms, shows a clear increase in microbial presence, bioalteration, and penetration into the rock substrate in older samples. The most significant visual changes appear to occur during the first years of exposure of the massif to atmospheric conditions. PB Springer Nature SN 1866-6280 SN 1866-6299 YR 2025 FD 2025-01-28 LK https://hdl.handle.net/20.500.14352/117180 UL https://hdl.handle.net/20.500.14352/117180 LA eng NO Iglesias-Martínez, M., Fernández-Suarez, J., de los Ríos, A. et al. Chromatic evolution, chemical changes, and biological colonisation in the quarry fronts of the Santullán limestone massif (Cantabria, Spain): implication for the mitigation of visual impact in mountain quarrying. Environ Earth Sci 84, 85 (2025). https://doi.org/10.1007/s12665-024-12008-z NO Instituto Geológico y Minero de España NO Consejo Superior de Investigaciones Científicas (España) NO Comunidad de Madrid DS Docta Complutense RD 18 abr 2025