RT Journal Article
T1 Contributing to the management of viral infections through simple immunosensing of the arachidonic acid serum level
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Iftimie, Simona
A1 Montero Calle, Ana
A1 Pingarrón Carrazón, José Manuel
A1 Castro, Antoni
A1 Camps, Jordi
A1 Barderas Manchado, Rodrigo
A1 Campuzano Ruiz, Susana
A1 Joven, Jorge
AB A trendsetting direct competitive-based biosensing tool has been developed and implemented for the determination of the polyunsaturated fatty acid arachidonic acid (ARA), a highly significant biological regulator with decisive roles in viral infections. The designed methodology involves a competitive reaction between the target endogenous ARA and a biotin-ARA competitor for the recognition sites of anti-ARA antibodies covalently attached to the surface of carboxylic acid-coated magnetic microbeads (HOOC-MμBs), followed by the enzymatic label of the biotin-ARA residues with streptavidin-horseradish peroxidase (Strep-HRP) conjugate. The resulting bioconjugates were magnetically trapped onto the sensing surface of disposable screen-printed carbon transducers (SPCEs) to monitor the extent of the biorecognition reaction through amperometry. The operational functioning of the exhaustively optimized and characterized immunosensing bioplatform was highly convenient for the quantitative determination of ARA in serum samples from severe acute respiratory syndrome coronavirus 2 (SARSCoV-2-) and respiratory syncytial virus (RSV)-infected individuals in a rapid, affordable, trustful, and sensitive manner.
PB Springer
YR 2024
FD 2024-06-04
LK https://hdl.handle.net/20.500.14352/123885
UL https://hdl.handle.net/20.500.14352/123885
LA eng
NO Torrente-Rodríguez, R.M., Ruiz-Valdepeñas Montiel, V., Iftimie, S. et al. Contributing to the management of viral infections through simple immunosensing of the arachidonic acid serum level. Microchim Acta 191, 369 (2024). https://doi.org/10.1007/s00604-024-06440-y
NO MCIN/AEI/https://doi.org/10.13039/501100011033
NO ERDF A way of making Europe
NO AES-ISCIII program
NO FEDER
NO La Fundació Marató de TV3
NO CRUE-CSIC
DS Docta Complutense
RD 27 mar 2026