RT Journal Article
T1 Antimicrobial activity, antibiotic susceptibility and virulence factors of Lactic Acid Bacteria of aquatic origin intended for use as probiotics in aquaculture
A1 Muñoz Atienza, Estefanía
A1 Gómez Sala, Beatriz
A1 Araújo, Carlos
A1 Campanero, Cristina
A1 Del Campo, Rosa
A1 Hernández Cruza, Pablo Elpidio
A1 Herranz Sorribes, Carmen
A1 Cintas Izarra, Luis Miguel
AB Background. The microorganisms intended for use as probiotics in aquaculture should exert antimicrobial activity and be regarded as safe not only for the aquatic hosts but also for their surrounding environments and humans. The objective of this work was to investigate the antimicrobial/bacteriocin activity against fish pathogens, the antibiotic susceptibility, and the prevalence of virulence factors and detrimental enzymatic activities in 99 Lactic Acid Bacteria (LAB) (59 enterococci and 40 non-enterococci) isolated from aquatic animals regarded as human food.Results. These LAB displayed a broad antimicrobial/bacteriocin activity against the main Gram-positive and Gram-negative fish pathogens. However, particular safety concerns based on antibiotic resistance and virulence factors were identified in the genus Enterococcus (86%) (Enterococcus faecalis, 100%; E. faecium, 79%). Antibiotic resistance was also found in the genera Weissella (60%), Pediococcus (44%), Lactobacillus (33%), but not in leuconostocs and lactococci. Antibiotic resistance genes were found in 7.5% of the non-enterococci, including the genera Pediococcus (12.5%) and Weissella (6.7%). One strain of both Pediococcus pentosaceus and Weissella cibaria carried the erythromycin resistance gene mef(A/E), and another two P. pentosaceus strains harboured lnu(A) conferring resistance to lincosamides. Gelatinase activity was found in E. faecalis and E. faecium (71 and 11%, respectively), while a low number of E. faecalis (5%) and none E. faecium exerted hemolytic activity. None enterococci and non-enterococci showed bile deconjugation and mucin degradation abilities, or other detrimental enzymatic activities.Conclusions. To our knowledge, this is the first description of mef(A/E) in the genera Pediococcus and Weissella, and lnu(A) in the genus Pediococcus. The in vitro subtractive screening presented in this work constitutes a valuable strategy for the large-scale preliminary selection of putatively safe LAB intended for use as probiotics in aquaculture.
PB BioMed Central. Part of of Springer Nature.
SN 1471-2180
YR 2013
FD 2013-01-24
LK https://hdl.handle.net/20.500.14352/92990
UL https://hdl.handle.net/20.500.14352/92990
LA eng
NO Ministerio de Ciencia y Tecnología (AGL2009−08348-ALI)
NO Universidad Complutense de Madrid/Banco Santander-Central Hispano (GR35/10-A)
NO Dirección General de Universidades e Investigación, Consejería de Educación, Comunidad de Madrid  (S−2009/AGR−1489)
NO Ministerio de Ciencia e Innovación (HP2008-0070)
DS Docta Complutense
RD 26 abr 2025