RT Journal Article
T1 Determination of immobilized lipase stability depends on the substrate and activity determination condition: Stress inactivations and optimal temperature as biocatalysts stability indicators
A1 da Rocha, Thays N.
A1 Carballares, Diego
A1 Guimarães, José R.
A1 Rocha Martín, Javier
A1 Tardioli, Paulo W.
A1 Gonçalves, Luciana R.B.
A1 Fernandez Lafuente, Roberto
AB Lipases A and B from Candida antarctica (CALA and CALB), Thermomyces lanuginosus (TLL) and Candida rugosa have been immobilized on octyl, octyl-vinyl sulfone (blocked with ethylendiamine) and amino-glutaraldehyde. The biocatalysts exhibited different specificity versus triacetin and p-nitro phenyl butyrate. Optimal activities were determined using triacetin for all biocatalysts, and this ranged from 40 °C for CALA and TLL, to 60 °C for an amino-glutaraldehyde-CRL. The biocatalysts were inactivated at 70 and 75 °C, determining their residual activities at 25 °C or 55 °C. The inactivation courses were very different depending on the substrate; in most cases the biocatalysts maintained more activity during the thermal inactivation using triacetin (except using TLL). When determining the residual activities at 55 °C, the values increased in most cases, reaching high hyperactivation values using CALA (even 23 folds). That way, the “stability” of the different preparations was strongly influenced by the substrate and residual activity determination conditions, and did not agree in most cases with the optimal temperatures.
PB Elsevier
YR 2022
FD 2022-10
LK https://hdl.handle.net/20.500.14352/124683
UL https://hdl.handle.net/20.500.14352/124683
LA eng
NO Da Rocha TN, Carballares D, Guimarães JR, Rocha-Martin J, Tardioli PW, Gonçalves LRB, Fernandez-Lafuente R. Determination of immobilized lipase stability depends on the substrate and activity determination condition: Stress inactivations and optimal temperature as biocatalysts stability indicators. Sustainable Chemistry and Pharmacy 2022;29:100823. https://doi.org/10.1016/j.scp.2022.100823.
NO AcknowledgmentsWe gratefully recognize the financial support from Ministerio de Ciencia e Innovación/Agencia Estatal de Investigación/10.13039/501100011033/(PID2021-122398OB-I00). DC thanks to Ministerio de Ciencia e Innovación-Spanish Government by a FPI (PRE2018-084809). The help and suggestions from Dr. Ángel Berenguer (Departamento de Química Inorgánica, Universidad de Alicante) are gratefully recognized. Thays Nogueira da Rocha thanks CNPq for the predoctoral fellowship (CNPq—Brazil).
NO Ministerio de Ciencia e Innovación (España)
NO Agencia Estatal de Investigación (España)
NO Conselho Nacional de Desenvolvimento Científico e Tecnológico (Brasil)
DS Docta Complutense
RD 20 ene 2026