RT Journal Article
T1 Effect of Pharmacological Inhibition of the Catalytic Activity of Phosphatases of Regenerating Liver in Early T Cell Receptor Signaling Dynamics and IL-2 Production
A1 Aguilar Sopeña, Óscar
A1 Hernández Pérez, Sara
A1 Alegre Gómez, Sergio
A1 Castro Sánchez, Patricia
A1 Iglesias Ceacero, Alba
A1 Lazo, John S.
A1 Roda Navarro, Pedro
AB We have previously shown the delivery of phosphatase of regenerating liver-1 (PRL-1) to the immunological synapse (IS) and proposed a regulatory role of the catalytic activity of PRLs (PRL-1, PRL-2 and PRL-3) in antigen-induced IL-2 production. Nonetheless, the expression in T cells and delivery to the IS of the highly homologous PRL-3, as well as the role of the catalytic activity of PRLs in antigen-induced early signaling, has not been investigated. Here, the expression of PRL-3 protein was detected in primary CD4 T cells and in the CD4 T cell line Jurkat (JK), in which an overexpressed GFP-PRL-3 fluorescent fusion protein trafficked through the endosomal recycling compartment and co-localized with PLCγ1 signaling sites at the IS. Pharmacological inhibition was used to compare the role of the catalytic activity of PRLs in antigen-induced early signaling and late IL-2 production. Although the phosphatase activity of PRLs was not critical for early signaling triggered by antigen, it seemed to regulate signaling dynamics and was necessary for proper IL-2 production. We propose that enzymatic activity of PRLs has a higher significance for cytokine production than for early signaling at the IS. However, further research will be necessary to deeply understand the regulatory role of PRLs during lymphocyte activation and effector function.
PB MDPI
SN 1422-0067
YR 2020
FD 2020-04-05
LK https://hdl.handle.net/20.500.14352/8197
UL https://hdl.handle.net/20.500.14352/8197
LA eng
NO Ministerio de Economía y Competitividad (MINECO)
DS Docta Complutense
RD 9 may 2025