RT Journal Article
T1 Ultrasound-assisted Co-extraction and characterization of saponin-rich Co-extracts from fenugreek seeds (Trigonella foenum-graecum L.) and quinoa husk (Chenopodium quinoa Willd.) and their hydrolysates
A1 Cantero Bahíllo, Emma
A1 Siles Sánchez, María de las Nieves
A1 Jaime, Laura
A1 Martín, Diana
A1 Navarro Del Hierro, Joaquín
AB The co-extraction of steroidal and triterpenoid saponins, followed by hydrolysis to produce sapogenin-rich extracts, offers an efficient strategy to enhance the multi-bioactive potential of extracts. The resulting chemical composition modifications, including the presence of other co-extracted or co-hydrolyzed compounds, must also be considered. In this study, ultrasound-assisted co-extraction of fenugreek seeds and quinoa husk using methanol and 50% aqueous methanol was conducted to obtain extracts and co-extracts with varying proportions of steroidal and triterpenoid saponins, in ratios of 0:100, 25:75, 50:50, 75:25, and 100:0, respectively. Aqueous methanol produced the highest extraction yields (15–25%) and saponin contents (40–70%) in the extracts and co-extracts. The conversion by microwave-assisted acid hydrolysis of saponins to sapogenins was more efficient for triterpenoid saponins, leading to a higher triterpenoid sapogenin content in all co-extracts, accounting for over 70% of the total sapogenins. The co-extracts also contained other bioactive compounds, such as phenolic compounds (3–4 g/100 g), with their content being favored by the presence of fenugreek. Hydrolysis altered the overall composition of the saponin-rich co-extracts, enriching the resulting hydrolyzed products in both types of sapogenins, along with fatty acids, carbonyls, non-protein nitrogen compounds and organic acids, while reducing monoglycerides, removing carbohydrates and significantly modifying the phenolic profile by eliminating many original phenolic compounds and generating new ones. Therefore, co-extraction and post-extraction hydrolysis of fenugreek and quinoa allowed the combination of triterpenoid and steroidal saponins or sapogenins into single products, along with other specific compounds from both sources.
PB Elsevier
SN 2212-4292
YR 2024
FD 2024-10-10
LK https://hdl.handle.net/20.500.14352/117966
UL https://hdl.handle.net/20.500.14352/117966
LA eng
NO Cantero-Bahillo, E., Siles-Sánchez, M. d. l. N., Jaime, L., Martin, D., & Navarro del Hierro, J. (2024). Ultrasound-assisted Co-extraction and characterization of saponin-rich Co-extracts from fenugreek seeds (Trigonella foenum-graecum L.) and quinoa husk (Chenopodium quinoa Willd.) and their hydrolysates. Food Bioscience, 62. https://doi.org/10.1016/J.FBIO.2024.105275
NO Author contributions:Emma Cantero-Bahillo: Writing – original draft, Methodology, Investigation, Formal analysis, Data curation, Conceptualization. María de las Nieves Siles-Sánchez: Methodology, Investigation, Formal analysis, Data curation. Laura Jaime: Writing – review & editing, Supervision, Project administration, Funding acquisition. Diana Martin: Writing – review & editing, Supervision, Project administration, Funding acquisition, Conceptualization. Joaquín Navarro del Hierro: Writing – review & editing, Supervision, Conceptualization.
NO Ministerio de Ciencia e Innovación (España)
DS Docta Complutense
RD 13 abr 2025