RT Journal Article T1 Corynebacterium conjunctivae: A New Corynebacterium Species Isolated from the Ocular Surface of Healthy Horses A1 LaFrentz, Stacey A1 Casamayor, Almudena A1 Abarca, Eva A1 Mohammed, Haitham A1 Cuming, Rosemary A1 Arias, Cova A1 Vela Alonso, Ana Isabel A1 Domínguez Rodríguez, Lucas José A1 Fernández-Garayzábal Fernández, José Francisco AB Twenty-two unidentified Gram-positive, rod-shaped organisms were recovered from the conjunctival surface of apparently healthy horses and subjected to a polyphasic taxonomic analysis. Based on cellular morphology and biochemical criteria, the isolates were tentatively assigned to the genus Corynebacterium, although they did not match any recognized species. Comparative 16S rRNA gene sequencing studies demonstrated that all of the isolates were phylogenetically members of the genus Corynebacterium. The isolates shared 99.4 to 100% 16S rRNA gene sequence similarity among the strains and 96.5% similarity with Corynebacterium tapiri 2385/12T, which was the closest phylogenetically related species. The DNA G+C content was 58.4 mol%. The major fatty acids were C15:0, C16:0, C17:1 ω8c and C18:1 ω9c, while the predominant mycolic acids consisted of C30:0, C32:0 and C34:0. The isolates were distinguished from related Corynebacterium species by a number of phenotypic properties. On the basis of phenotypic and phylogenetic evidence, it is proposed that the unknown isolates from horses be classified in the genus Corynebacterium as Corynebacterium conjunctivae sp. nov. The type strain of C. conjunctivae is ICM19-01138T (DSM 109759T = CCUG 73728T). PB MPDI SN 2076-2615 YR 2022 FD 2022-07-18 LK https://hdl.handle.net/20.500.14352/73218 UL https://hdl.handle.net/20.500.14352/73218 LA eng NO Ministerio de Ciencia, Innovación y Universidades (MCIU) DS Docta Complutense RD 21 abr 2025