RT Journal Article
T1 Comparison of Different Strategies for the Development of Highly Sensitive Electrochemical Nucleic Acid Biosensors Using Neither Nanomaterials nor Nucleic Acid Amplification
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Povedano Muñumel, Eloy
A1 Vargas, Eva
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Pedrero Muñoz, María
A1 Reviejo García, Ángel Julio
A1 Campuzano Ruiz, Susana
A1 Pingarrón Carrazón, José Manuel
AB Currently, electrochemical nucleic acid-based biosensing methodologies involving hybridization assays, specific recognition of RNA/DNA and RNA/RNAduplexes, and amplification systems provide an attractive alternative to conventional quantification strategies for the routine determination of relevant nucleic acids at different settings. A particularly relevant objective in the development of such nucleic acid biosensors is the design of as many as possible affordable, quick, and simple methods while keeping the required sensitivity. With this aim in mind, this work reports, for the first time, a thorough comparison between 11 methodologies that involve different assay formats and labeling strategies for targeting the same DNA. The assayed approaches use conventional sandwich and competitive hybridization assays, direct hybridization coupled to bioreceptors with affinity for RNA/DNA duplexes, multienzyme labeling bioreagents, and DNA concatamers. All of them have been implemented on the surface of magnetic beads (MBs) and involve amperometrictransduction at screen-printed carbon electrodes (SPCEs). The influence of the formed duplex length and of the labeling strategy have also been evaluated. Results demonstrate that these strategies can provide very sensitive methods without the need for using nanomaterials or polymerase chain reaction (PCR). In addition, the sensitivity can be tailored within several orders of magnitude simply by varying the bioassay format, hybrid length or labeling strategy. This comparative study allowed us to conclude that the use of strategies involving longer hybrids, the use of antibodies with specificity for RNA/DNA heteroduplexes and labeling with bacterial antibody binding proteins conjugated with multiple enzyme molecules, provides the best sensitivity.
PB American Chemical Society
SN 2379-3694
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/95003
UL https://hdl.handle.net/20.500.14352/95003
LA eng
NO Ruiz-Valdepeñas Montiel V, Povedano E, Vargas E, Torrente-Rodríguez RM, Pedrero M, Reviejo AJ, Campuzano S, Pingarrón JM. Comparison of Different Strategies for the Development of Highly Sensitive Electrochemical Nucleic Acid Biosensors Using Neither Nanomaterials nor Nucleic Acid Amplification. ACS Sens. 2018 Jan 26;3(1):211-221. doi: 10.1021/acssensors.7b00869. Epub 2018 Jan 16. PMID: 29282977. Fo
NO Ministerio de Economía y Competitividad (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 9 abr 2025