RT Journal Article
T1 Enhancement of Cryo-EM maps by a multiscale tubular filter
A1 Vargas Balbuena, Javier
A1 Gómez Pedrero, José Antonio
A1 Quiroga Mellado, Juan Antonio
A1 Alonso Fernández, José
AB We present an approach to enhance cryo-electron microscopy (cryo-EM) postprocessed maps based on a multiscale tubular filter. The method determines a tubularness measure locally by the analysis of the eigenvalues of the Hessian matrix. This information is used to enhance elongated local structures and to attenuate blob-like and plate-like structures. The approach, thus, introduces a priori information in the reconstructions to improve their interpretability and analysis at high-resolution. The proposed method has been tested with simulated and real cryo-EM maps including recent reconstructions of the SARS-CoV-2. Our results show that our methods can improve obtained reconstructions.
PB The Optical Society of America
SN 1094-4087
YR 2022
FD 2022-01-31
LK https://hdl.handle.net/20.500.14352/71328
UL https://hdl.handle.net/20.500.14352/71328
LA eng
NO © 2022 Optica Publishing Group.Funding. Ministerio de Ciencia e Innovación (PID2019-108850RA-I00).
NO Ministerio de Ciencia e Innovacion (MICINN)
DS Docta Complutense
RD 8 abr 2025