RT Journal Article T1 Wastewater-based epidemiology as a surveillance tool to assess human consumption of psychotropic substances: Alcohol, nicotine and caffeine as case studies A1 Rousis, Nikolaos A1 Bade, Richard A1 Gracia Lor, Emma AB Wastewater-based epidemiology (WBE) is used as a surveillance tool to provide data on public health, drug use, exposure to chemicals and lifestyle habits. This approach can be used to monitor the achievement of the United Nations Sustainable Development Goals, such as reducing alcohol consumption and reducing the use of tobacco. In addition, it can be applied to monitor total caffeine consumption and assess levels according to the recommended daily safety limit as suggested by the European Food Safety Authority. The purpose of this review was to summarize and discuss analytical protocols for the determination of alcohol, nicotine, and caffeine WBE biomarkers in wastewater. The WBE requirements for these biomarkers were thoroughly investigated. Furthermore, back-calculated levels of alcohol, nicotine, and caffeine in the population were reviewed. Finally, challenges were identified, and future directions were proposed to fill knowledge gaps and improve the quality of information obtained from the WBE approach. PB Elsevier YR 2023 FD 2023-08 LK https://hdl.handle.net/20.500.14352/114227 UL https://hdl.handle.net/20.500.14352/114227 LA eng NO Nikolaos Rousis, Richard Bade, Emma Gracia-Lor, Wastewater-based epidemiology as a surveillance tool to assess human consumption of psychotropic substances: Alcohol, nicotine and caffeine as case studies, TrAC Trends in Analytical Chemistry, Volume 167, 2023, 117230, ISSN 0165-9936, https://doi.org/10.1016/j.trac.2023.117230. NO Plan Nacional sobre Drogas (Ministerio de Sanidad) NO Comunidad de Madrid NO Gobierno australiano DS Docta Complutense RD 7 abr 2025