RT Journal Article
T1 Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells
A1 Sánchez Beltrán, María Del Carmen
A1 Romero Lastra, Patricia
A1 Ribeiro Vidal, Honorato
A1 Llama Palacios, María Arantxazu
A1 Figuero Ruiz, Elena
A1 Herrera González, David
A1 Sanz Alonso, Mariano
AB Background: Porphyromonas gingivalis, a microorganism residing in the oral cavity within complex multispecies biofilms, is one of the keystone pathogens in the onset and progression of periodontitis. In this in vitro study, using DNA microarray, we investigate the differential gene expression of Porphyromonas gingivalis ATCC 33277 when growing in the presence or in absence of its own monospecies biofilm.Results: Approximately 1.5% of genes (28 out of 1909 genes, at 1.5 fold change or more, p-value < 0.05) were differentially expressed by P. gingivalis cells when in the presence of a biofilm. These genes were predominantly related to the metabolism of iron, bacterial adhesion, invasion, virulence and quorum-sensing system. The results from microarrays were consistent with those obtained by RT-qPCR.Conclusion: This study provides insight on the transcriptional changes of planktonic P. gingivalis cells when growing in the presence of a biofilm. The resulting phenotypes provide information on changes occurring in the gene expression of this pathogen.
PB BMC: BioMed Central
SN 1471-2180
YR 2019
FD 2019
LK https://hdl.handle.net/20.500.14352/94749
UL https://hdl.handle.net/20.500.14352/94749
LA eng
NO Sánchez MC, Romero-Lastra P, Ribeiro-Vidal H, Llama-Palacios A, Figuero E, Herrera D, Sanz M. Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells. BMC Microbiol. 2019 Mar 12;19(1):58
DS Docta Complutense
RD 18 abr 2025