RT Journal Article
T1 Recognition of peptidoglycan and beta-lactam antibiotics by the extracellular domain of the Ser/Thr protein kinase StkP from Streptococcus pneumoniae
A1 Maestro García-Donas, María Beatriz
A1 Novakova, Linda
A1 Hesek, Dusan
A1 Lee, Mijoo
A1 Leyva, Eduardo
A1 Mobashery, Shahriar
A1 Sanz, Jesús
A1 Branny, Pavel
AB The eukaryotic-type serine/threonine kinase StkP from Streptococcus pneumoniae is an important signal-transduction element that regulates the expression of numerous pneumococcal genes. We have expressed the extracellular C-terminal domain of StkP kinase (C-StkP), elaborated a three-dimensional structural model and performed a spectroscopical characterization of its structure and stability. Biophysical experiments show that C-StkP binds to synthetic samples of the cell wall peptidoglycan (PGN) and to β-lactam antibiotics, which mimic the terminal portions of the PGN stem peptide. This is the first experimental report on the recognition of a minimal PGN unit by a PASTA-containing kinase, suggesting that non-crosslinked PGN may act as a signal for StkP function and pointing to this protein as an interesting target for β-lactam antibiotics.
PB Wiley
SN 0014-5793
YR 2010
FD 2010
LK https://hdl.handle.net/20.500.14352/93182.2
UL https://hdl.handle.net/20.500.14352/93182.2
LA eng
NO Maestro, Beatriz, et al. «Recognition of Peptidoglycan and β-Lactam Antibiotics by the Extracellular Domain of the Ser/Thr Protein Kinase StkP from Streptococcus Pneumoniae». FEBS Letters, vol. 585, n.o 2, enero de 2011, pp. 357-63. https://doi.org/10.1016/j.febslet.2010.12.016.
NO European Commission
NO Czech Science Foundation
NO Agency of the Academy of Sciences of the Czech Republic
NO Institutional Research Concept
NO National Institutes of Health for the research in the USA
DS Docta Complutense
RD 15 dic 2025