RT Journal Article
T1 Surface functionalisation with viscosity-sensitive BODIPY molecular rotor
A1 López Duarte, Ismael
A1 Aurimas Vyšniauskas, 
A1 Alex J Thompson, 
A1 James A Bull, 
A1 Marina K Kuimova, 
AB Surface functionalisation with viscosity sensitive dyes termed 'molecular rotors' can potentially open up new opportunities in sensing, for example for non-invasive biological viscosity imaging, in studying the effect of shear stress on lipid membranes and in cells, and in imaging contacts between surfaces upon applied pressure. We have functionalised microscope slides with BODIPY-based molecular rotor capable of viscosity sensing via its fluorescence lifetime. We have optimised functionalisation conditions and prepared the slides with the BODIPY rotor attached directly to the surface of glass slides and through polymer linkers of 5 kDa and 40 kDa in mass. The slides were characterised for their sensitivity to viscosity, and used to measure viscosity of supported lipid bilayers during photooxidation, and of giant unilamellar vesicles lying on the surface of the slide. We conclude that our functionalised slides show promise for a variety of viscosity sensing applications.
PB IOP Publishing
SN 2050-6120
YR 2018
FD 2018-04-13
LK https://hdl.handle.net/20.500.14352/115189
UL https://hdl.handle.net/20.500.14352/115189
LA eng
NO Vyšniauskas A, Lopez-Duarte I, Thompson AJ, Bull JA, Kuimova MK. Surface functionalisation with viscosity-sensitive BODIPY molecular rotor. Methods Appl Fluoresc 2018;6:034001. https://doi.org/10.1088/2050-6120/aabb2c.
NO Career Acceleration Fellowship (EPSRC)
DS Docta Complutense
RD 15 may 2026