RT Journal Article
T1 An Electroanalytical Approach for Advancing Cancer Diagnosis and Therapy by Tracking Globally DNA G‑Quadruplex Noncanonical Structures
A1 Cabrero Martín, Andrea
A1 Ruiz Valdepeñas Montiel, Víctor
A1 Torrente Rodríguez, Rebeca Magnolia
A1 Povedano Muñumel, Eloy
A1 Velázquez Gutiérrez, Javier
A1 Garranzo Asensio, María
A1 Pingarrón Carrazón, José Manuel
A1 Barderas Manchado, Rodrigo
A1 Campuzano Ruiz, Susana
AB This work reports the first electroanalytical methodology developed to determine global levels of G-quadruplex (G4) motifs in DNA, hitherto underestimated noncanonical structures of increasing relevance both in cancer diagnostics and precision therapy. The methodology developed takes advantage of the benefits provided by magnetic microcarriers to implement a competitive immunoassay involving a synthetic DNA sequence carrying a single G4 motif and a commercial recombinant antibody that can recognize these motifs. This antibody was enzymatically labeled with an HRP-conjugated secondary antibody, and amperometric transduction was performed using disposable electrode substrates. The careful selection of the bioassay format and the optimization of the experimental variables has led to a groundbreaking technology allowing the determination in only 10 min of synthetic sequences carrying a single G4 motif at the low nM level. The methodology was applied to the analysis of 50 ng of genomic DNA extracted from different cancer cells, highlighting its potential to accurately determine the content of such structures in these extracts and to relate their expression to the silencing of key genes or to the cell’s ability to interact with potential oncotherapeutic agents capable of stabilizing these noncanonical structures.
PB ACS Publications
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/134199
UL https://hdl.handle.net/20.500.14352/134199
LA eng
NO Cabrero-Martín, A., Ruiz-Valdepeñas Montiel, V., Torrente-Rodríguez, R. M., Povedano, E., Velázquez-Gutiérrez, J., Garranzo-Asensio, M., ... & Campuzano, S. (2025). An Electroanalytical Approach for Advancing Cancer Diagnosis and Therapy by Tracking Globally DNA G-Quadruplex Noncanonical Structures. Analytical Chemistry, 97(36), 19560-19569.
NO Agencia Estatal de Investigación (España)
NO Ministerio de Ciencia e Inovación (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 25 mar 2026