%0 Journal Article
%A Rivero, Cintia Wanda
%A García, Natalia Soledad
%A Fernández Lucas, Jesús
%A Betancor, Lorena
%A Romanelli, Gustavo Pablo
%A Trelles, Jorge Abel
%T Green production of cladribine by using immobilized 2'-deoxyribosyltransferase from Lactobacillus delbrueckii stabilized through a double covalent/entrapment Technology
%D 2021
%U https://hdl.handle.net/20.500.14352/109033
%X Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end, Lactobacillus delbrueckii NDT (LdNDT) was successfully immobilized through a two-step immobilization methodology, including a covalent immobilization onto glutaraldehyde-activated biomimetic silica nanoparticles followed by biocatalyst entrapment in calcium alginate. The resulting immobilized derivative, SiGPEI 25000-LdNDT-Alg, displayed 98% retained activity and was shown to be active and stable in a broad range of pH (5–9) and temperature (30–60 °C), but also displayed an extremely high reusability (up to 2100 reuses without negligible loss of activity) in the enzymatic production of cladribine. Finally, as a proof of concept, SiGPEI 25000-LdNDT-Alg was successfully employed in the green production of cladribine at mg scale.
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