RT Journal Article
T1 Green production of cladribine by using immobilized 2'-deoxyribosyltransferase from Lactobacillus delbrueckii stabilized through a double covalent/entrapment Technology
A1 Rivero, Cintia Wanda
A1 García, Natalia Soledad
A1 Fernández Lucas, Jesús
A1 Betancor, Lorena
A1 Romanelli, Gustavo Pablo
A1 Trelles, Jorge Abel
AB Nowadays, enzyme-mediated processes offer an eco-friendly and efficient alternative to the traditional multistep and environmentally harmful chemical processes. Herein we report the enzymatic synthesis of cladribine by a novel 2′-deoxyribosyltransferase (NDT)-based combined biocatalyst. To this end, Lactobacillus delbrueckii NDT (LdNDT) was successfully immobilized through a two-step immobilization methodology, including a covalent immobilization onto glutaraldehyde-activated biomimetic silica nanoparticles followed by biocatalyst entrapment in calcium alginate. The resulting immobilized derivative, SiGPEI 25000-LdNDT-Alg, displayed 98% retained activity and was shown to be active and stable in a broad range of pH (5–9) and temperature (30–60 °C), but also displayed an extremely high reusability (up to 2100 reuses without negligible loss of activity) in the enzymatic production of cladribine. Finally, as a proof of concept, SiGPEI 25000-LdNDT-Alg was successfully employed in the green production of cladribine at mg scale.
PB MDPI
YR 2021
FD 2021
LK https://hdl.handle.net/20.500.14352/109033
UL https://hdl.handle.net/20.500.14352/109033
LA eng
NO Rivero CW, García NS, Fernández-Lucas J, Betancor L, Romanelli GP, Trelles JA. Green Production of Cladribine by Using Immobilized 2′-Deoxyribosyltransferase from Lactobacillus delbrueckii Stabilized through a Double Covalent/Entrapment Technology. Biomolecules 2021;11:657. https://doi.org/10.3390/biom11050657
NO This research was mainly supported by Agencia Nacional de Promoción Científica y Tecnológica (PICT 2014–3438), Consejo Nacional de Investigaciones Científicas y Técnicas (PIP 2014- 11220130100805CO) and Universidad Nacional de Quilmes (PUNQ 1305/19, PPROF 2017-EXPTE.895/18)
NO Agencia Nacional de Promoción Científica y Tecnológica (Argentina)
NO Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina)
NO Universidad Nacional de Quilmes
NO Fundación  Santander
NO Universidad Europea de Madrid
DS Docta Complutense
RD 6 abr 2025