RT Journal Article
T1 Identification of Structural and Molecular Signatures Mediating Adaptive Changes in the Mouse Kidney in Response to Pregnancy
A1 Lopez-Tello, Jorge
A1 Jiménez Martínez, María de los Ángeles
A1 Salazar-Petres, Esteban
A1 Patel, Ritik
A1 George, Amy
A1 Kay, Richard
A1 Sferruzzi-Perri, Amanda
AB Pregnancy is characterized by adaptations in the function of several maternal body systems that ensure the development of the fetus whilst maintaining health of the mother. The renal system is responsible for water and electrolyte balance, as well as waste removal. Thus, it is imperative that structural and functional changes occur in the kidney during pregnancy. However, our knowledge of the precise morphological and molecular mechanisms occurring in the kidney during pregnancy is still very limited. Here, we investigated the changes occurring in the mouse kidney during pregnancy by performing an integrated analysis involving histology, gene and protein expression assays, mass spectrometry profiling and bioinformatics. Data from non-pregnant and pregnant mice were used to identify critical signalling pathways mediating changes in the maternal kidneys. We observed an expansion of renal medulla due to proliferation and infiltration of interstitial cellular constituents, as well as alterations in the activity of key cellular signalling pathways (e.g., AKT, AMPK and MAPKs) and genes involved in cell growth/metabolism (e.g., Cdc6, Foxm1 and Rb1) in the kidneys during pregnancy. We also generated plasma and urine proteomic profiles, identifying unique proteins in pregnancy. These proteins could be used to monitor and study potential mechanisms of renal adaptations during pregnancy and disease.
PB MDPI
SN 1661-6596
YR 2022
FD 2022
LK https://hdl.handle.net/20.500.14352/95696
UL https://hdl.handle.net/20.500.14352/95696
LA eng
NO Author ContributionsConceived and designed the study, J.L.-T. and A.N.S.-P.; animal work, J.L.-T.; investigation and data curation, J.L.-T., M.A.J.-M., E.S.-P., R.P., A.L.G., R.G.K. and A.N.S.-P.; formal analysis, J.L.-T., E.S.-P., A.L.G. and R.G.K.; visualization, J.L.-T.; writing—original draft preparation, J.L.-T. and A.N.S.-P.; review and editing, all authors. All authors have read and agreed to the published version of the manuscript.
NO Wellcome Trust
NO Lister Institute
DS Docta Complutense
RD 17 abr 2025