RT Journal Article
T1 Effect of 16h of rabbit sperm incubation on capacitation and heterologous in vitro fertilization
A1 Ruiz-Díaz, Sara
A1 Maroto, María
A1 Fernández-Gonzalez, Raúl
A1 de Frutos, Celia
A1 Lombó, Marta
A1 Arias Álvarez, María
AB In vitro fertilization (IVF) in rabbits is an important biotechnology, but its success remains low due to inefficient sperm capacitation. This study examines relationships between motility parameters (MP), protein tyrosine phosphorylation (PTP) and acrosomal exocytosis (AE) between sperm recovered after direct swim-up (DSU), and after 16 h of in vitro incubation under capacitating conditions, and heterologous IVF capacity. Variables were assessed in fresh ejaculates (ES), and after 2 h of DSU, and 16 h (T16) of incubation. PTP were grouped into four patterns in the different sperm structures according to their time-dependent appearance and AE. Throughout incubation, MP remained stable whereas AE increased after DSU and 16 h of incubation. Mid-piece PTP patterns increased after DSU and persisted until 16 h in reacted (R) and non-reacted spermatozoa (NR). In R and NR, annulus-ring PTP levels were also significantly higher in T16 versus DSU or ES. Flagellum PTP levels rose significantly during incubation such that highest levels were recorded in T16. In R sperm, levels of PTP patterns associated with the equatorial zone in T16 were significantly higher compared to NR and to DSU and ES. Cleavage rates were significantly higher at T16 than DSU and these were similar to rates recorded for homologous IVF used as positive control. Our capacitation protocol and PTP patterns at 16 h rendered the sperm competent to fertilize an oocyte in vitro. This time point matches the physiological timing of fertilization in vivo. We also describe a new annulus ring PTP pattern for this species.
PB Elsevier
SN 0378-4320
YR 2025
FD 2025
LK https://hdl.handle.net/20.500.14352/122216
UL https://hdl.handle.net/20.500.14352/122216
LA eng
NO Ruiz-Díaz, S., Maroto, M., Fernández-Gonzalez, R., de Frutos, C., Lombó, M., & Arias-Álvarez, M. (2025). Effect of 16h of rabbit sperm incubation on capacitation and heterologous in vitro fertilization. Animal reproduction science, 277, 107841. https://doi.org/10.1016/j.anireprosci.2025.107841
NO Justificación de autores:Fernandez Gonzalez Raul: Writing – review & editing, Methodology, Investigation. Maroto Maria: Writing – review & editing, Methodology, Investigation. Arias Maria: Writing – review & editing, Writing – original draft, Funding acquisition, Formal analysis, Conceptualization. Lombó Marta: Writing – review & editing, Methodology, Investigation. de Frutos Celia: Writing – review & editing, Methodology, Investigation. Ruiz Diaz Sara: Writing – review & editing, Writing – original draft, Methodology, Investigation, Formal analysis, Data curation, Conceptualization.Becas:FJC2019-040385-IIND2018/BIO-9610
NO European Commission
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Comunidad de Madrid
DS Docta Complutense
RD 11 jul 2025