RT Journal Article
T1 Autophagy impairment aggravates the inhibitory effects of high glucose on osteoblast viability and function
A1 Bartolomé, Alberto
A1 López-Herradón, Ana
A1 Portal Nuñez, Sergio
A1 García Aguilar, Ana
A1 Esbrit, Pedro
A1 Gómez Hernández, María De La Almudena
A1 Benito De Las Heras, Manuel R.
A1 Guillén Viejo, Carlos
AB Autophagy is a highly regulated homoeostatic process involved in the lysosomal degradation of damaged cell organelles and proteins. This process is considered an important pro-survival mechanism under diverse stress conditions. A diabetic milieu is known to hamper osteoblast viability and function. In the present study, we explored the putative protective role of autophagy in osteoblastic cells exposed to an HG (high glucose) medium. HG was found to increase protein oxidation and triggered autophagy by a mechanism dependent on reactive oxygen species overproduction in osteoblastic MC3T3-E1 cells. MC3T3-E1 cell survival was impaired by HG and worsened by chemical or genetic inhibition of autophagy. These findings were mimicked by H2O2-induced oxidative stress in these cells. Autophagy impairment led to both defective mitochondrial morphology and decreased bioenergetic machinery and inhibited further osteoblast differentiation in MC3T3-E1 cells upon exposure to HG. These novel findings indicate that autophagy is an essential mechanism to maintain osteoblast viability and function in an HG environment.
PB Portland Press Ltd.
SN 0264-6021
YR 2013
FD 2013
LK https://hdl.handle.net/20.500.14352/115677
UL https://hdl.handle.net/20.500.14352/115677
LA eng
NO Bartolomé A, López-Herradón A, Portal-Núñez S, García-Aguilar A, Esbrit P, Benito M, Guillén C. Autophagy impairment aggravates the inhibitory effects of high glucose on osteoblast viability and function. Biochem J. 2013 Nov 1;455(3):329-37. doi: 10.1042/BJ20130562. PMID: 23981124.
NO Fundación Mutua Madrileña
NO Fundación Conchita Rábago
NO Red Tematica de Investigación Cooperativa en Envejecimiento y Fragilidad
NO European Commission
NO Ministerio de Ciencia, Innovación y Universidades (España)
NO Ministerio de Educación, Cultura y Deporte (España)
NO Instituto de Salud Carlos III
DS Docta Complutense
RD 11 abr 2025