RT Journal Article
T1 In vitro and in vivo effect of flutamide on steroid hormone secretion in canine and human inflammatory breast cancer cell lines
A1 Cáceres Ramos, Sara Cristina
A1 Monsalve, Beatriz
A1 Peña Fernández, Laura Luisa
A1 Andrés Gamazo, Paloma Jimena de
A1 Alonso‐Diez, Ángela
A1 Illera del Portal, Josefina María
A1 Woodward, Wendy
A1 Reuben, James
A1 Silván Granado, Gema
A1 Illera Del Portal, Juan Carlos
AB The aim was to study the effects of flutamide on cell proliferation, in vivo tumour growth andsteroid production in canine and human IBC cell lines. IPC-366 and SUM149 cell cultures wereexposed to flutamide concentrations for 72 hours. Additionally, IPC-366 and SUM149 xeno-transplanted mice were treated subcutaneously with flutamide 3 times a week for 2 weeks.Steroid hormones determination in culture media, serum and tumour homogenates (pregneno-lone, progesterone, androstenedione, testosterone, dihydrotestosterone, 17β-oestradiol andoestrone sulphate) were assayed by EIA. in vitro cell proliferation percentages showed adecrease in all flutamide dosages in IPC-366 and SUM149. in vivo flutamide reduced tumoursize by 55% to 65%, and metastasis rates decreased. In treated groups, androgen levels in cul-ture media, serum and tumour homogenates were increased as oestrogen levels decreased. These results suggest that flutamide treatment inhibits cell proliferation and promotes tumourreduction by increasing androgen levels and also support future therapy approaches
PB Wiley
SN 1476-5810
YR 2017
FD 2017
LK https://hdl.handle.net/20.500.14352/95543
UL https://hdl.handle.net/20.500.14352/95543
LA eng
NO Caceres S, Monsalve B, Peña L, de Andres PJ, Alonso-Diez A, Illera MJ, Woodward WA, Reuben JM, Silvan G, Illera JC. In vitro and in vivo effect of flutamide on steroid hormone secretion in canine and human inflammatory breast cancer cell lines. Vet Comp Oncol. 2018 Mar;16(1):148-158. doi: 10.1111/vco.12324. Epub 2017 Jun 6. PMID: 28589573.
NO National Cancer Institute
DS Docta Complutense
RD 21 abr 2025