RT Journal Article
T1 Regenerative neurogenic response from glia requires insulin-driven neuron-glia communication
A1 Harrison, Neale
A1 Connolly, Elizabeth
A1 Gascón Gunieda, Alicia
A1 Yang, Zidan
A1 Altenhein, Benjamin
A1 Losada Pérez, María De La Paloma
A1 Moreira, Marta
A1 Sun, Jun
A1 Hidalgo, Alicia
AB Understanding how injury to the central nervous system induces de novo neurogenesis in animals would help promote regeneration in humans. Regenerative neurogenesis could originate from glia and glial neuron-glia antigen-2 (NG2) may sense injury-induced neuronal signals, but these are unknown. Here, we used Drosophila to search for genes functionally related to the NG2 homologue kon-tiki (kon), and identified Islet Antigen-2 (Ia-2), required in neurons for insulin secretion. Both loss and over-expression of ia-2 induced neural stem cell gene expression, injury increased ia-2 expression and induced ectopic neural stem cells. Using genetic analysis and lineage tracing, we demonstrate that Ia-2 and Kon regulate Drosophila insulin-like peptide 6 (Dilp-6) to induce glial proliferation and neural stem cells from glia. Ectopic neural stem cells can divide, and limited de novo neurogenesis could be traced back to glial cells. Altogether, Ia-2 and Dilp-6 drive a neuron-glia relay that restores glia and reprogrammes glia into neural stem cells for regeneration.
PB eLife Sciences Publications
YR 2021
FD 2021-02-02
LK https://hdl.handle.net/20.500.14352/105285
UL https://hdl.handle.net/20.500.14352/105285
LA eng
NO Harrison, N. J., Connolly, E., Gascón Gubieda, A., Yang, Z., Altenhein, B., Losada Perez, M., Moreira, M., Sun, J., & Hidalgo, A. (2021). Regenerative neurogenic response from glia requires insulin-driven neuron-glia communication. eLife, 10, e58756. https://doi.org/10.7554/eLife.58756
NO Biotechnology and Biological Sciences Research Council
NO MSCA
DS Docta Complutense
RD 11 abr 2025