RT Journal Article
T1 Prevalence, Distribution and Antimicrobial Susceptibility of Enterobacteriaceae and Non-Fermenting Gram-Negative Bacilli Isolated From Environmental Samples in a Veterinary Clinical Hospital in Madrid, Spain
A1 Pérez Jiménez, Jesús Antonio
A1 Penelo Hidalgo, Silvia
A1 Baquero Artigao, María Rosario
A1 Ortiz Díez, Gustavo
A1 Ayllón Santiago, Tania
AB Managing infections caused by multidrug-resistant Gram-negative bacilli is a major public health concern, particularly in hospitals where surfaces can act as reservoirs for resistant microorganisms. Identifying these bacteria in hospital environments is crucial for improving healthcare safety. This study aimed to analyse environmental samples from a veterinary hospital to identify prevalent microorganisms and detect antimicrobial resistance patterns. A total of 183 surface samples were collected from 26 areas at the Veterinary Clinical Hospital of Alfonso X el Sabio University in Madrid. The isolated strains were identified, and susceptibility profiles were determined via the disk diffusion method. Clonality analysis was performed using pulsed-field gel electrophoresis. In total, 109 strains were isolated: 76.15% from the Enterobacteriaceae family and 23.85% non-fermenting Gram-negative bacilli. The isolates included Klebsiella, Enterobacter, Escherichia and Pseudomonas species, which could include high-risk clones, given their ability to carry several antimicrobial resistance genes. The equine area had the highest number of isolates (n = 71), accounting for 65% of the total. High resistance indices were observed against at least five of the 16 antibiotics tested, indicating significant multidrug resistance. Clonality analysis suggested potential cross-transmission within the facility. This study sampled hospital surfaces but not personnel or animals, making contamination sources unclear. Without resampling, the effectiveness of cleaning protocols remains uncertain. Results suggest that hospital staff play a key role in bacterial transmission. The lack of specialised preventive measures in veterinary hospitals highlights a need for further research and improvement
PB Wiley
SN 1758-2229
YR 2024
FD 2024
LK https://hdl.handle.net/20.500.14352/113495
UL https://hdl.handle.net/20.500.14352/113495
LA eng
NO Pérez Jiménez, J. A., Penelo Hidalgo, S., Baquero Artigao, M.-R., Ortiz-Díez, G., & Ayllón Santiago, T. (2024). Prevalence, Distribution and Antimicrobial Susceptibility of Enterobacteriaceae and Non-Fermenting Gram-Negative Bacilli Isolated From Environmental Samples in a Veterinary Clinical Hospital in Madrid, Spain. Environmental Microbiology Reports, 16(6). https://doi.org/10.1111/1758-2229.70055
NO Author Contributions:Jesús Antonio Pérez Jiménez: methodology, formal analysis, inves tigation, data curation, writing – review and editing. Silvia Penelo  Hidalgo: validation, data curation, writing – original draft, writing – review and editing, visualization. María-Rosario Baquero Artigao: conceptualization, resources, supervision, project administration, fund ing acquisition. Gustavo Ortiz-Díez: software, formal analysis, data curation, writing – original draft, validation. Tania Ayllón Santiago: validation, data curation, writing – original draft, writing – review and  editing, visualization, supervision.
NO Fundación Universitaria Alfonso X el Sabio
NO Banco  Santander
DS Docta Complutense
RD 6 abr 2025